Detection of iprobenfos and edifenphos using a new multi-aptasensor

Young Seop Kwon; Van Thuan Nguyen; Je Gun Park; Man Bock Gu

doi:10.1016/j.aca.2015.02.020

Detection of iprobenfos and edifenphos using a new multi-aptasensor

Young Seop Kwon, Van Thuan Nguyen, Je Gun Park, Man Bock Gu

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

Abstract

The sensitive detection of iprobenfos (IBF) and edifenphos (EDI) was successfully conducted by using a new aptamer-based colorimetric multi-aptasensor. The dissociation constants of this multi-target aptamer to both iprobenfos and edifenphos were found to be 1.67. μM and 38. nM, respectively, according to the isothermal calorimetry assay. The aptamer EIA2 was selective to only IBF and EDI, confirmed by AuNP assays. By using this multi-aptasensor, both pesticides IBF and EDI can be eventually detected in a range from 10. nM to 5. nM, respectively. This multi-aptasensor was successfully implemented in spiked rice samples and the accuracies of this AuNP-based multi-aptasensor were around 80 and 90% in spiked paddy and polished rice samples, respectively. This aptamer EIA2 could be applied not only for the detection of pesticides from real samples in agriculture field as POC, but also can be used as a bioreceptor for other types of aptasensors.

Original language	English
Pages (from-to)	60-66
Number of pages	7
Journal	Analytica Chimica Acta
Volume	868
DOIs	https://doi.org/10.1016/j.aca.2015.02.020
Publication status	Published - 2015 Apr 8

Keywords

Atpamer
Colorimetric
Edifenphos
Iprobenfos
Multi-aptasensor

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy

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10.1016/j.aca.2015.02.020

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title = "Detection of iprobenfos and edifenphos using a new multi-aptasensor",

abstract = "The sensitive detection of iprobenfos (IBF) and edifenphos (EDI) was successfully conducted by using a new aptamer-based colorimetric multi-aptasensor. The dissociation constants of this multi-target aptamer to both iprobenfos and edifenphos were found to be 1.67. μM and 38. nM, respectively, according to the isothermal calorimetry assay. The aptamer EIA2 was selective to only IBF and EDI, confirmed by AuNP assays. By using this multi-aptasensor, both pesticides IBF and EDI can be eventually detected in a range from 10. nM to 5. nM, respectively. This multi-aptasensor was successfully implemented in spiked rice samples and the accuracies of this AuNP-based multi-aptasensor were around 80 and 90% in spiked paddy and polished rice samples, respectively. This aptamer EIA2 could be applied not only for the detection of pesticides from real samples in agriculture field as POC, but also can be used as a bioreceptor for other types of aptasensors.",

keywords = "Atpamer, Colorimetric, Edifenphos, Iprobenfos, Multi-aptasensor",

author = "Kwon, {Young Seop} and Nguyen, {Van Thuan} and Park, {Je Gun} and Gu, {Man Bock}",

note = "Funding Information: This research was supported by Advanced Production Technology Development Program , Ministry of Agriculture, Food and Rural Affairs and the National Research Foundation of Korea (NRF) grant funded by the Korean Government (No. 2013R1A1A2021531 ). Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

year = "2015",

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AU - Kwon, Young Seop

AU - Nguyen, Van Thuan

AU - Park, Je Gun

AU - Gu, Man Bock

N1 - Funding Information: This research was supported by Advanced Production Technology Development Program , Ministry of Agriculture, Food and Rural Affairs and the National Research Foundation of Korea (NRF) grant funded by the Korean Government (No. 2013R1A1A2021531 ). Publisher Copyright: © 2015 Elsevier B.V.

PY - 2015/4/8

Y1 - 2015/4/8

N2 - The sensitive detection of iprobenfos (IBF) and edifenphos (EDI) was successfully conducted by using a new aptamer-based colorimetric multi-aptasensor. The dissociation constants of this multi-target aptamer to both iprobenfos and edifenphos were found to be 1.67. μM and 38. nM, respectively, according to the isothermal calorimetry assay. The aptamer EIA2 was selective to only IBF and EDI, confirmed by AuNP assays. By using this multi-aptasensor, both pesticides IBF and EDI can be eventually detected in a range from 10. nM to 5. nM, respectively. This multi-aptasensor was successfully implemented in spiked rice samples and the accuracies of this AuNP-based multi-aptasensor were around 80 and 90% in spiked paddy and polished rice samples, respectively. This aptamer EIA2 could be applied not only for the detection of pesticides from real samples in agriculture field as POC, but also can be used as a bioreceptor for other types of aptasensors.

AB - The sensitive detection of iprobenfos (IBF) and edifenphos (EDI) was successfully conducted by using a new aptamer-based colorimetric multi-aptasensor. The dissociation constants of this multi-target aptamer to both iprobenfos and edifenphos were found to be 1.67. μM and 38. nM, respectively, according to the isothermal calorimetry assay. The aptamer EIA2 was selective to only IBF and EDI, confirmed by AuNP assays. By using this multi-aptasensor, both pesticides IBF and EDI can be eventually detected in a range from 10. nM to 5. nM, respectively. This multi-aptasensor was successfully implemented in spiked rice samples and the accuracies of this AuNP-based multi-aptasensor were around 80 and 90% in spiked paddy and polished rice samples, respectively. This aptamer EIA2 could be applied not only for the detection of pesticides from real samples in agriculture field as POC, but also can be used as a bioreceptor for other types of aptasensors.

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Detection of iprobenfos and edifenphos using a new multi-aptasensor

Abstract

Keywords

ASJC Scopus subject areas

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