Detection of iprobenfos and edifenphos using a new multi-aptasensor

Young Seop Kwon, Van Thuan Nguyen, Je Gun Park, Man Bock Gu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The sensitive detection of iprobenfos (IBF) and edifenphos (EDI) was successfully conducted by using a new aptamer-based colorimetric multi-aptasensor. The dissociation constants of this multi-target aptamer to both iprobenfos and edifenphos were found to be 1.67. μM and 38. nM, respectively, according to the isothermal calorimetry assay. The aptamer EIA2 was selective to only IBF and EDI, confirmed by AuNP assays. By using this multi-aptasensor, both pesticides IBF and EDI can be eventually detected in a range from 10. nM to 5. nM, respectively. This multi-aptasensor was successfully implemented in spiked rice samples and the accuracies of this AuNP-based multi-aptasensor were around 80 and 90% in spiked paddy and polished rice samples, respectively. This aptamer EIA2 could be applied not only for the detection of pesticides from real samples in agriculture field as POC, but also can be used as a bioreceptor for other types of aptasensors.

Original languageEnglish
Pages (from-to)60-66
Number of pages7
JournalAnalytica Chimica Acta
Volume868
DOIs
Publication statusPublished - 2015 Apr 8

Fingerprint

rice
pesticide
assay
calorimetry
Pesticides
Assays
agriculture
Calorimetry
Agriculture
detection
O,O-diisopropyl-S-benzylthiophosphate
edifenphos
Oryza

Keywords

  • Atpamer
  • Colorimetric
  • Edifenphos
  • Iprobenfos
  • Multi-aptasensor

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Detection of iprobenfos and edifenphos using a new multi-aptasensor. / Kwon, Young Seop; Nguyen, Van Thuan; Park, Je Gun; Gu, Man Bock.

In: Analytica Chimica Acta, Vol. 868, 08.04.2015, p. 60-66.

Research output: Contribution to journalArticle

Kwon, Young Seop ; Nguyen, Van Thuan ; Park, Je Gun ; Gu, Man Bock. / Detection of iprobenfos and edifenphos using a new multi-aptasensor. In: Analytica Chimica Acta. 2015 ; Vol. 868. pp. 60-66.
@article{2f45b930809c441a9ed7807463336ef1,
title = "Detection of iprobenfos and edifenphos using a new multi-aptasensor",
abstract = "The sensitive detection of iprobenfos (IBF) and edifenphos (EDI) was successfully conducted by using a new aptamer-based colorimetric multi-aptasensor. The dissociation constants of this multi-target aptamer to both iprobenfos and edifenphos were found to be 1.67. μM and 38. nM, respectively, according to the isothermal calorimetry assay. The aptamer EIA2 was selective to only IBF and EDI, confirmed by AuNP assays. By using this multi-aptasensor, both pesticides IBF and EDI can be eventually detected in a range from 10. nM to 5. nM, respectively. This multi-aptasensor was successfully implemented in spiked rice samples and the accuracies of this AuNP-based multi-aptasensor were around 80 and 90{\%} in spiked paddy and polished rice samples, respectively. This aptamer EIA2 could be applied not only for the detection of pesticides from real samples in agriculture field as POC, but also can be used as a bioreceptor for other types of aptasensors.",
keywords = "Atpamer, Colorimetric, Edifenphos, Iprobenfos, Multi-aptasensor",
author = "Kwon, {Young Seop} and Nguyen, {Van Thuan} and Park, {Je Gun} and Gu, {Man Bock}",
year = "2015",
month = "4",
day = "8",
doi = "10.1016/j.aca.2015.02.020",
language = "English",
volume = "868",
pages = "60--66",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",

}

TY - JOUR

T1 - Detection of iprobenfos and edifenphos using a new multi-aptasensor

AU - Kwon, Young Seop

AU - Nguyen, Van Thuan

AU - Park, Je Gun

AU - Gu, Man Bock

PY - 2015/4/8

Y1 - 2015/4/8

N2 - The sensitive detection of iprobenfos (IBF) and edifenphos (EDI) was successfully conducted by using a new aptamer-based colorimetric multi-aptasensor. The dissociation constants of this multi-target aptamer to both iprobenfos and edifenphos were found to be 1.67. μM and 38. nM, respectively, according to the isothermal calorimetry assay. The aptamer EIA2 was selective to only IBF and EDI, confirmed by AuNP assays. By using this multi-aptasensor, both pesticides IBF and EDI can be eventually detected in a range from 10. nM to 5. nM, respectively. This multi-aptasensor was successfully implemented in spiked rice samples and the accuracies of this AuNP-based multi-aptasensor were around 80 and 90% in spiked paddy and polished rice samples, respectively. This aptamer EIA2 could be applied not only for the detection of pesticides from real samples in agriculture field as POC, but also can be used as a bioreceptor for other types of aptasensors.

AB - The sensitive detection of iprobenfos (IBF) and edifenphos (EDI) was successfully conducted by using a new aptamer-based colorimetric multi-aptasensor. The dissociation constants of this multi-target aptamer to both iprobenfos and edifenphos were found to be 1.67. μM and 38. nM, respectively, according to the isothermal calorimetry assay. The aptamer EIA2 was selective to only IBF and EDI, confirmed by AuNP assays. By using this multi-aptasensor, both pesticides IBF and EDI can be eventually detected in a range from 10. nM to 5. nM, respectively. This multi-aptasensor was successfully implemented in spiked rice samples and the accuracies of this AuNP-based multi-aptasensor were around 80 and 90% in spiked paddy and polished rice samples, respectively. This aptamer EIA2 could be applied not only for the detection of pesticides from real samples in agriculture field as POC, but also can be used as a bioreceptor for other types of aptasensors.

KW - Atpamer

KW - Colorimetric

KW - Edifenphos

KW - Iprobenfos

KW - Multi-aptasensor

UR - http://www.scopus.com/inward/record.url?scp=84925292637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84925292637&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2015.02.020

DO - 10.1016/j.aca.2015.02.020

M3 - Article

C2 - 25813235

AN - SCOPUS:84925292637

VL - 868

SP - 60

EP - 66

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

ER -