Detection of leptospiral DNA by PCR

Sun-Ho Kee, I. S. Kim, M. S. Choi, W. H. Chang

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

An EcoRI fragment (1.2 kb) which is highly conserved among Leptospira interrogans isolated in Korea was cloned into pBluescript vector from L. interrogans serovar lai WH20. The EcoRI fragment was sequenced, and a pair of primers (LP1 and LP2) was designed for PCR assay. PCR amplification of target DNA obtained from cultured L. interrogans showed that 274 bp could be detected when as little as 100 fg of leptospiral genomic DNA was used in the reaction mixture. No amplification of DNA was detected from DNA of Leptospira biflexa serovars patoc and sau paulo, Borrelia burgdorferi, Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium. Amplification of 274- bp target DNA could be detected in DNA samples purified from 500 μl of blood collected from experimentally infected gerbils 2 days after infection, while antibodies to L. interrogans could be detected by the microscopic agglutination test 7 days after infection. The specificity and high sensitivity of the test provided valuable tools for the early diagnosis of leptospirosis.

Original languageEnglish
Pages (from-to)1035-1039
Number of pages5
JournalJournal of Clinical Microbiology
Volume32
Issue number4
Publication statusPublished - 1994 Jan 1
Externally publishedYes

Fingerprint

Leptospira interrogans
Polymerase Chain Reaction
DNA
Leptospira
Agglutination Tests
Leptospirosis
Borrelia burgdorferi
Gerbillinae
Salmonella typhimurium
Korea
Infection
Staphylococcus aureus
Early Diagnosis
Escherichia coli
Sensitivity and Specificity
Antibodies
Serogroup

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Kee, S-H., Kim, I. S., Choi, M. S., & Chang, W. H. (1994). Detection of leptospiral DNA by PCR. Journal of Clinical Microbiology, 32(4), 1035-1039.

Detection of leptospiral DNA by PCR. / Kee, Sun-Ho; Kim, I. S.; Choi, M. S.; Chang, W. H.

In: Journal of Clinical Microbiology, Vol. 32, No. 4, 01.01.1994, p. 1035-1039.

Research output: Contribution to journalArticle

Kee, S-H, Kim, IS, Choi, MS & Chang, WH 1994, 'Detection of leptospiral DNA by PCR', Journal of Clinical Microbiology, vol. 32, no. 4, pp. 1035-1039.
Kee S-H, Kim IS, Choi MS, Chang WH. Detection of leptospiral DNA by PCR. Journal of Clinical Microbiology. 1994 Jan 1;32(4):1035-1039.
Kee, Sun-Ho ; Kim, I. S. ; Choi, M. S. ; Chang, W. H. / Detection of leptospiral DNA by PCR. In: Journal of Clinical Microbiology. 1994 ; Vol. 32, No. 4. pp. 1035-1039.
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