Detection of metastatic tumors after γ-irradiation using longitudinal molecular imaging and gene expression profiling of metastatic tumor nodules

Su Jin Jang, Joo Hyun Kang, Yong Jin Lee, Kwang Il Kim, Tae Sup Lee, Jae-Gol Choe, Sang Moo Lim

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A few recent reports have indicated that metastatic growth of several human cancer cells could be promoted by radiotherapy. C6-L cells expressing the firefly luciferase (fLuc) gene were implanted subcutaneously into the right thigh of BALB/c nu/nu mice. C6-L xenograft mice were treated locally with 50-Gy γ-irradiation (γ-IR) in five 10-Gy fractions. Metastatic tumors were evaluated after γ-IR by imaging techniques. Total RNA from non-irradiated primary tumor (NRPT), γ-irradiated primary tumor (RPT), and three metastatic lung nodule was isolated and analyzed by microarray. Metastatic lung nodules were detected by BLI and PET/CT after 6-9 weeks of γ-IR in 6 (17.1%) of the 35 mice. The images clearly demonstrated high [18F]FLT and [18F]FDG uptake into metastatic lung nodules. Whole mRNA expression patterns were analyzed by microarray to elucidate the changes among NRPT, RPT and metastatic lung nodules after γ-IR. In particular, expression changes in the cancer stem cell markers were highly significant in RPT. We observed the metastatic tumors after γ-IR in a tumor-bearing animal model using molecular imaging methods and analyzed the gene expression profile to elucidate genetic changes after γ-IR.

Original languageEnglish
Pages (from-to)1361-1368
Number of pages8
JournalInternational Journal of Oncology
Volume48
Issue number4
DOIs
Publication statusPublished - 2016 Apr 1

Keywords

  • aldehyde dehydrogenases
  • gene expression profiling
  • metastatic tumors
  • molecular imaging
  • γ-irradiation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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