Detection of microRNA as novel biomarkers of epithelial ovarian cancer from the serum of ovarian cancer patient

Ye Won Chung, Hyo Sook Bae, Jae Yun Song, Jae Kwan Lee, Nak Woo Lee, Tak Kim, Kyu Wan Lee

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Objective: MicroRNA (miRNA) is an abundant class of small noncoding RNAs that act as gene regulators. Recent studies have suggested thatmiRNA deregulation is associated with the initiation and progression of human cancer. However, information about cancer-related miRNA is mostly limited to tissue miRNA. The aim of this study was to find specific profiles of serum-derived miRNAsof ovarian cancer based on a comparative study using amiRNA microarray of serum, tissue, and ascites. Methods: From 2 ovarian cancer patients and a healthy control, total RNAwas isolated from their serum, tissue, and ascites, respectively, and analyzed by a microarray. Under the comparative study of eachmiRNAmicroarray,we sorted out severalmiRNAs showing a consistent regulation tendency throughout all 3 specimens and the greatest range of alteration in serum as potential biomarkers. The availability of biomarkerswas confirmed by qRT-PCRof 18 patients and 12 controls. Results: Out of 2222 kinds of total miRNAs that were identified in the microarray analysis, 95 miRNAs were down-regulated and 88 miRNAs were up-regulated, in the serum, tissue, and ascites of cancer patients. Among the miRNAs that showed a consistent regulation tendency through all specimens and showed more than a 2-fold difference in serum, 5 miRNAs (miR-132, miR-26a, let-7b, miR-145, and miR-143) were determined as the 5 most markedly down-regulated miRNAs in the serum from ovarian cancer patients with respect to those of controls. Four miRNAs (miR-132, miR-26a, let-7b, and miR-145) out of 5 selected miRNAs were significantly underexpressed in the serum of ovarian cancer patients in qRT-PCR. Conclusions: Serum miR-132, miR-26a, let-7b, and miR-145 could be considered as potential candidates as novel biomarkers in serous ovarian cancer. Also, serum miRNAs is a promising and useful tool for discriminating between controls and patientswith serous ovarian cancer.

Original languageEnglish
Pages (from-to)673-679
Number of pages7
JournalInternational Journal of Gynecological Cancer
Volume23
Issue number4
DOIs
Publication statusPublished - 2013 May 1

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MicroRNAs
Ovarian Neoplasms
Biomarkers
Serum
Ascites
Ovarian epithelial cancer
Small Untranslated RNA
Neoplasms
Regulator Genes
Microarray Analysis
Polymerase Chain Reaction

Keywords

  • Biological markers
  • MicroRNAs
  • Ovarian neoplasms

ASJC Scopus subject areas

  • Oncology
  • Obstetrics and Gynaecology

Cite this

Detection of microRNA as novel biomarkers of epithelial ovarian cancer from the serum of ovarian cancer patient. / Chung, Ye Won; Bae, Hyo Sook; Song, Jae Yun; Lee, Jae Kwan; Lee, Nak Woo; Kim, Tak; Lee, Kyu Wan.

In: International Journal of Gynecological Cancer, Vol. 23, No. 4, 01.05.2013, p. 673-679.

Research output: Contribution to journalArticle

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AU - Bae, Hyo Sook

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AU - Lee, Jae Kwan

AU - Lee, Nak Woo

AU - Kim, Tak

AU - Lee, Kyu Wan

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AB - Objective: MicroRNA (miRNA) is an abundant class of small noncoding RNAs that act as gene regulators. Recent studies have suggested thatmiRNA deregulation is associated with the initiation and progression of human cancer. However, information about cancer-related miRNA is mostly limited to tissue miRNA. The aim of this study was to find specific profiles of serum-derived miRNAsof ovarian cancer based on a comparative study using amiRNA microarray of serum, tissue, and ascites. Methods: From 2 ovarian cancer patients and a healthy control, total RNAwas isolated from their serum, tissue, and ascites, respectively, and analyzed by a microarray. Under the comparative study of eachmiRNAmicroarray,we sorted out severalmiRNAs showing a consistent regulation tendency throughout all 3 specimens and the greatest range of alteration in serum as potential biomarkers. The availability of biomarkerswas confirmed by qRT-PCRof 18 patients and 12 controls. Results: Out of 2222 kinds of total miRNAs that were identified in the microarray analysis, 95 miRNAs were down-regulated and 88 miRNAs were up-regulated, in the serum, tissue, and ascites of cancer patients. Among the miRNAs that showed a consistent regulation tendency through all specimens and showed more than a 2-fold difference in serum, 5 miRNAs (miR-132, miR-26a, let-7b, miR-145, and miR-143) were determined as the 5 most markedly down-regulated miRNAs in the serum from ovarian cancer patients with respect to those of controls. Four miRNAs (miR-132, miR-26a, let-7b, and miR-145) out of 5 selected miRNAs were significantly underexpressed in the serum of ovarian cancer patients in qRT-PCR. Conclusions: Serum miR-132, miR-26a, let-7b, and miR-145 could be considered as potential candidates as novel biomarkers in serous ovarian cancer. Also, serum miRNAs is a promising and useful tool for discriminating between controls and patientswith serous ovarian cancer.

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