Detection of nickel in fish organs with a two-photon fluorescent probe

Min Young Kang, Chang Su Lim, Hyun Soo Kim, Eun Won Seo, Hwan Myung Kim, Ohyun Kwon, Bong-Rae Cho

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Molecular imaging by two-photon microscopy (TPM) has become indispensable to the study of biology/medicine owing to its capability of imaging deep inside intact tissues. To make TPM a more-versatile tool, a large variety of two-photon probes are needed. Herein, we report a new two-photon fluorescent probe (ANi2) that can be excited by 750 nm femtosecond pulses and detect Ni 2+ ions in fresh fish organs at 90-175 μm depth without interference from the pH value or from other biologically relevant species through the use of TPM. TPM images of fish organs labeled with ANi2 revealed that Ni 2+ ions accumulate in fish organs in the order: kidney > heart > gill ≥ liver. Moreover, a linear relationship was found between the two-photon-excited fluorescence (TPEF) and the inductively coupled plasma mass spectrometry intensities (ICP-MS), thereby allowing the quantitative measurement of Ni 2+ ions in live tissue.

Original languageEnglish
Pages (from-to)1953-1960
Number of pages8
JournalChemistry - A European Journal
Volume18
Issue number7
DOIs
Publication statusPublished - 2012 Feb 13

Fingerprint

Nickel
Fluorescent Dyes
Photons
Fish
Fishes
Microscopy
Microscopic examination
Ions
Tissue
Molecular imaging
Inductively coupled plasma mass spectrometry
Molecular Imaging
Ultrashort pulses
Liver
Medicine
Mass Spectrometry
Fluorescence
Kidney
Imaging techniques

Keywords

  • fish
  • fluorescent probes
  • live tissue
  • nickel
  • two-photon microscopy

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Detection of nickel in fish organs with a two-photon fluorescent probe. / Kang, Min Young; Lim, Chang Su; Kim, Hyun Soo; Seo, Eun Won; Kim, Hwan Myung; Kwon, Ohyun; Cho, Bong-Rae.

In: Chemistry - A European Journal, Vol. 18, No. 7, 13.02.2012, p. 1953-1960.

Research output: Contribution to journalArticle

Kang, Min Young ; Lim, Chang Su ; Kim, Hyun Soo ; Seo, Eun Won ; Kim, Hwan Myung ; Kwon, Ohyun ; Cho, Bong-Rae. / Detection of nickel in fish organs with a two-photon fluorescent probe. In: Chemistry - A European Journal. 2012 ; Vol. 18, No. 7. pp. 1953-1960.
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