Detection of oyster-associated norovirus by microchip electrophoresis of an amplified cDNA

Ho Kyung Oh, Yeong Min Sin, Ki Hyun Kim, Kun Sang Park, Dae Byung Kim, Byung-Yoon Ahn, Ok Hee Kim

Research output: Contribution to journalArticle

Abstract

Noroviruses, members of the family Caliciviridae, are often found in shellfish grown in polluted water and are emerging as a leading cause of foodbome disease worldwide. As the presence of norovirus in food commodities becomes an important medical and social issue, there are increasing needs for designing improved detection methods for the virus. In this study, we tested the Agilent 2100 Bioanalyzer for the analysis of norovirus DNA amplified from oyster samples. Microchip electrophoresis provided us with more accurate information, compared to conventional agarose gel electrophoresis, in the resolution and quantification of amplified products. The development of an improved method for food-associated noroviruses would contribute to a rapid identification of contaminated food and improve our understanding of the modes of food contamination and norovirus transmission.

Original languageEnglish
Pages (from-to)126-130
Number of pages5
JournalJournal of Food Science and Nutrition
Volume12
Issue number2
DOIs
Publication statusPublished - 2007 Dec 1

Fingerprint

Microchip Electrophoresis
Norovirus
Ostreidae
oysters
electrophoresis
Complementary DNA
food contamination
Food
Caliciviridae
Food Contamination
commodity foods
Shellfish
Agar Gel Electrophoresis
shellfish
agarose
gel electrophoresis
Viruses
viruses
Water
DNA

Keywords

  • Bioanalyzer
  • Microchip electrophoresis
  • Norovirus
  • Oysters
  • RT-PCR
  • Viral gastroenteritis

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Detection of oyster-associated norovirus by microchip electrophoresis of an amplified cDNA. / Oh, Ho Kyung; Sin, Yeong Min; Kim, Ki Hyun; Park, Kun Sang; Kim, Dae Byung; Ahn, Byung-Yoon; Kim, Ok Hee.

In: Journal of Food Science and Nutrition, Vol. 12, No. 2, 01.12.2007, p. 126-130.

Research output: Contribution to journalArticle

Oh, Ho Kyung ; Sin, Yeong Min ; Kim, Ki Hyun ; Park, Kun Sang ; Kim, Dae Byung ; Ahn, Byung-Yoon ; Kim, Ok Hee. / Detection of oyster-associated norovirus by microchip electrophoresis of an amplified cDNA. In: Journal of Food Science and Nutrition. 2007 ; Vol. 12, No. 2. pp. 126-130.
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