Detection of Plasmodium vivax infection in the Republic of Korea by loop-mediated isothermal amplification (LAMP)

Jun Hu Chen, Feng Lu, Chae Seung Lim, Jung Yeon Kim, Heui June Ahn, In Bum Suh, Satoru Takeo, Takafumi Tsuboi, Jetsumon Sattabongkot, Eun Taek Han

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel technique that rapidly amplifies target DNA in isothermal conditions. In a previous study, the sensitivities and specificities of LAMP, microscopy, and nested PCR were compared in the context of rapid malaria detection. In the present study, LAMP detected vivax malaria parasites in 115 of 117 microscopically positive samples (sensitivity, 98.3%; 95% CI, 97.4-100%), which agreed well with the nested PCR results (sensitivity, 99.1%; 95% CI: 96.0-100%). No positive cases of malaria were detected by LAMP or nested PCR in 50 consecutive feverish patients other than malaria from malaria endemic areas. LAMP performed on DNA extracted from heat-treated blood had a sensitivity of 93.3% (28/30, 95% CI: 84.4-100%) and specificity of 100% (30/30, 95% CI: 100%). The present study shows that LAMP based assays have high sensitivity, specificity, and amplification efficiencies for Plasmodium vivax detection. The authors recommend that LAMP can be considered as a rapid nucleic acid amplification assay for the molecular diagnosis of P. vivax in both clinical laboratories and malaria clinics in areas where vivax malaria is endemic.

Original languageEnglish
Pages (from-to)61-65
Number of pages5
JournalActa Tropica
Volume113
Issue number1
DOIs
Publication statusPublished - 2010 Jan

Keywords

  • Diagnosis
  • LAMP
  • Malaria
  • Plasmodium vivax
  • Republic of Korea

ASJC Scopus subject areas

  • Parasitology
  • veterinary (miscalleneous)
  • Insect Science
  • Infectious Diseases

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