Detection of platelet-monocyte aggregates by the ADAM® image cytometer

Bo Kyeung Jung, Chi Hyun Cho, Kyung Chul Moon, Dae Sung Hur, Jeong Ah Yoon, Soo Young Yoon

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Background: Inappropriate platelet activation is known to be associated with various thrombotic disorders. Platelet-monocyte aggregates (PMAs), whose formation is mediated by platelet surface P-selectin (CD62P), can be used as a reliable marker to detect platelet activation. Previous studies have generally detected PMAs through flow cytometry-based approaches. Recently, the ADAM® image cytometer (Nanoentek Inc., Seoul, Korea) was developed for image-based cellular analysis. In this study, we detected PMAs with the ADAM® cytometer, evaluated the reproducibility of the measurements made by the ADAM® cytometer, and compared the abilities of the ADAM® cytometer and a flow cytometric assay to detect PMAs.

Methods: Whole blood samples were collected from patients. Within 5 minutes of collection, anticoagulated whole blood samples were fixed in 10% paraformaldehyde and 5% glyoxal. Nineteen clinical specimens were collected; each was analyzed three times with the ADAM® cytometer in order to assess the reproducibility of its measurements. To compare the ability of the ADAM® cytometer with that of a flow cytometer to detect PMAs, each cytometer was used for 23 clinical samples and the correlation of the measurements was determined.

Results: The PMA measurements made by the ADAM® cytometer showed good reproducibility (CV < 10% for all specimens). Moreover, the PMA measurements made by the ADAM® cytometer exhibited a high correlation with those made by a flow cytometric assay (R = 0.944).

Conclusions: The ADAM® cytometer is a suitable alternative method to the flow cytometry-based assays. Since the ADAM cytometer does not need specialized instrument knowledge or software proficiency (unlike flow cytometry), the ADAM® cytometer can be used as a rapid and reliable POCT device to measure platelet activation in peripheral blood. This, in turn, will provide valuable information regarding patient propensities to thrombotic diseases.

Original languageEnglish
Pages (from-to)1228-1233
Number of pages6
JournalInternational Journal of Medical Sciences
Volume11
Issue number12
DOIs
Publication statusPublished - 2014 Sep 18

Keywords

  • ADAM®
  • Image cytometer
  • Platelet activation
  • Platelet-monocyte aggregates

ASJC Scopus subject areas

  • Medicine(all)

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