Detection of post-translational modifications by comparison of intact proteins with tryptic digests in a complex biological sample

P. Kristina Taylor, Sang Won Lee, Christophe Masselon, Ljiljana Pasa-Tolic, Deanna L. Auberry, Mary S. Lipton, Richard D. Smith

Research output: Contribution to conferencePaperpeer-review

Abstract

Post-translational modifications were detected by comparison of intact proteins with tryptic digests in a complex biological sample. Bicinchoninic Acid (BCA) protein assay was utilized to determine protein concentration of samples for intact protein analysis. Final concentration was determined by BCA assay. The intact protein masses were examined to determine possible phosphorylated proteins that corresponded to proteins identified through enzymatic digestion. It was found that only a subset of proteins present in the sample was used to identify the intact protein masses, which reduces the number of mistaken identifications.

Original languageEnglish
Pages157-158
Number of pages2
Publication statusPublished - 2002
Externally publishedYes
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: 2002 Jun 22002 Jun 6

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period02/6/202/6/6

ASJC Scopus subject areas

  • Spectroscopy

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