Post-translational modifications were detected by comparison of intact proteins with tryptic digests in a complex biological sample. Bicinchoninic Acid (BCA) protein assay was utilized to determine protein concentration of samples for intact protein analysis. Final concentration was determined by BCA assay. The intact protein masses were examined to determine possible phosphorylated proteins that corresponded to proteins identified through enzymatic digestion. It was found that only a subset of proteins present in the sample was used to identify the intact protein masses, which reduces the number of mistaken identifications.
|Number of pages||2|
|Publication status||Published - 2002|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2002 Jun 2 → 2002 Jun 6
|Other||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||02/6/2 → 02/6/6|
ASJC Scopus subject areas