Detection of radiation effects using recombinant bioluminescent Escherichia coli strains

Jiho Min, Chang Woo Lee, Seung Hyeun Moon, Robert A. LaRossa, Man Bock Gu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Effects of ionizing radiation (0.1-500 Gy) on recombinant Escherichia coli cells containing the stress promoters recA, grpE, or katG, fused to luxCDABE, were characterized by monitoring transcriptional responses reflected by the bioluminescent output. The minimum dose of gamma-irradiation detected by E. coli DPD2794 (recA::IuxCDABE) was about 1.5 Gy, while the maximum response was obtained at 200 Gy. The amount of emitted bioluminescence increased proportionally with the gamma-ray doses which were found to elicit a DNA damage response in a range of 1-50 Gy. In addition, the cell growth rate was severely, but transiently, retarded by about 50 Gy. Quantification of the gamma-ray dose may be possible using the recA promoter fusion, since linear enhancement of the bioluminescence emission with increasing gamma-ray dose was observed. Other irradiated strains (50 Gy) responsive to either oxidative stress (DPD2511, katG::luxCDABE) or protein- damaging stress (TV1061, grpE::luxCDABE) did not display an increased bioluminescent output, while DPD2794 irradiated by the same dose of gamma- rays gave a significant bioluminescent output. This indicates that the recA promoter is the one most suitable for developing a biosensor for ionizing radiation.

Original languageEnglish
Pages (from-to)41-45
Number of pages5
JournalRadiation and Environmental Biophysics
Volume39
Issue number1
DOIs
Publication statusPublished - 2000 Mar

Keywords

  • Bioluminescent bacteria
  • DNA damage
  • Ionizing radiation biosensor
  • Oxidative damage
  • Protein damage
  • Stress promoters

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Environmental Science(all)

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