Detection of t(11;22)(q24;q12) translocation of Ewing's sarcoma in paraffin embedded tissue by nested reverse transcription-polymerase chain reaction

Yong Koo Park, Sung-Gil Chi, Hye Rim Park, Moon Ho Yang, K. Krishnan Unni

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ewing's sarcoma is a poorly characterized malignant tumor with a relatively uniform histologic appearance, made up of densely packed small cells with round to oval nuclei, without distinct cell borders and without any structural differentiation. Often the diagnosis has to be made by exclusion. Recently, it has been made possible to identify characteristic chromosomal rearrangements associated with certain solid tumors. More than 85% of Ewing's sarcoma and peripheral neuroectodermal tumor present a specific t(11;22)(q24;q12) balanced translocation, resulting in the production of a novel chimerical EWS/FLI-1 message. Using oligonudeotide primers derived from EWS and FLI-1 complementary DNAs, we were able to use reverse transcription-polymerase chain reaction (RT-PCR) as a diagnostic tool. The described nested RT-PCR method as another supportive diagnostic method enables pathologists to differentiate small blue cell tumors not only to make correct diagnosis but also to investigate retrospective archival tumor samples, using formalin fixed paraffin embedded tissue as a source of RNA. Copyright & The Korean Academy of Medical Sciences.

Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalJournal of Korean Medical Science
Volume13
Issue number4
Publication statusPublished - 1998 Aug 1
Externally publishedYes

    Fingerprint

Keywords

  • Nested RT-PCR
  • Sarcoma, Ewing's
  • Translocation, genetic, t(11;22)(q24;q12)

ASJC Scopus subject areas

  • Medicine(all)

Cite this