Detection of VR-2332 strain of porcine reproductive and respiratory syndrome virus type II using an aptamer-based sandwich-type assay

Su Jin Lee, Young Seop Kwon, Ji Eun Lee, Eun Jin Choi, Chang Hee Lee, Jae Young Song, Man Bock Gu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome disease (PRRS), a disease that has a significant and economic impact on the swine industry. In this study, single-stranded DNA (ssDNA) aptamers with high specificity and affinity against VR-2332 strain of PRRSV type II were successfully obtained. Of 19 candidates, the LB32 aptamer was found to be the most specific and sensitive to VR-2332 strain according to an aptamer-based surface plasmon resonance (SPR) analysis. The detection of VR-2332 of PRRSV type II was successfully accomplished using the enzyme-linked antibody-aptamer sandwich (ELAAS) method. The detection limit of ELAAS was 4.8 × 100 TCID50/mL that is comparable to some of the previous reports of the PCR-based detection but does not require any complicated equipment or extra costs. Moreover, this ELAAS-based PRRSV detection showed similar sensitivity for both the VR-2332 samples spiked in diluted swine serum and in buffer. Therefore, this VR-2332 strain-specific aptamer and its assay method with high specificity can be used as an alternative method for the fast and precise detection of PRRSV.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalAnalytical Chemistry
Volume85
Issue number1
DOIs
Publication statusPublished - 2013 Jan 2

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Viruses
Assays
Antibodies
Enzymes
Nucleotide Aptamers
Pulmonary diseases
Single-Stranded DNA
Surface plasmon resonance
Buffers
Economics
Costs
Industry

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Detection of VR-2332 strain of porcine reproductive and respiratory syndrome virus type II using an aptamer-based sandwich-type assay. / Lee, Su Jin; Kwon, Young Seop; Lee, Ji Eun; Choi, Eun Jin; Lee, Chang Hee; Song, Jae Young; Gu, Man Bock.

In: Analytical Chemistry, Vol. 85, No. 1, 02.01.2013, p. 66-74.

Research output: Contribution to journalArticle

Lee, Su Jin ; Kwon, Young Seop ; Lee, Ji Eun ; Choi, Eun Jin ; Lee, Chang Hee ; Song, Jae Young ; Gu, Man Bock. / Detection of VR-2332 strain of porcine reproductive and respiratory syndrome virus type II using an aptamer-based sandwich-type assay. In: Analytical Chemistry. 2013 ; Vol. 85, No. 1. pp. 66-74.
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