Determination of fluorescent whitening agents in paper materials by ion-pair reversed-phase high-performance liquid chromatography

Jeong Soo Kim, Do Hwan Kim, Keon Kim

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10 Citations (Scopus)

Abstract

A simple method was developed for the analysis of seven stilbene-type fluorescent whitening agents (FWAs) in paper materials by ion-pair reversed-phase high-performance liquid chromatography with fluorescence detection. These stilbene-type FWAs included two disulfonate, two tetrasulfonate, and three hexasulfonate compounds. After optimization of chromatographic conditions, the FWAs were satisfactorily separated using a reversed-phase column (RP-18) with the following isocratic mobile phase: methanol-water (60:40) containing 17.5 mM TBABr and 10 mM citrate buffer (pH = 7.0). The calibration plot was linear in the range from 5 to 500 ng/mL for two disulfo-FWAs and from 1 to 500 ng/mL for the other five FWAs. Precision levels of the calibration curve as indicated by RSD of response factors were 1.2 and 8.1%. Limits of quantitation (LOQ) ranged from 1.2 to 11 ng/mL.

Original languageEnglish
Pages (from-to)3971-3976
Number of pages6
JournalBulletin of the Korean Chemical Society
Volume33
Issue number12
DOIs
Publication statusPublished - 2012 Dec 20

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Keywords

  • Fluorescent whitening agents
  • Ion-pair chromatography
  • Paper materials

ASJC Scopus subject areas

  • Chemistry(all)

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