Determination of functional domains in polypyrimidine-tract-binding protein

Young L. Oh, Bumsuk Hahm, Yoon Ki Kim, Hae K. Lee, Joo W. Lee, Ok K. Song, Kyoko Tsukiyama-Kohara, Michinori Kohara, Akio Nomoto, Sung K. Jang

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Polypyrimidine-tract-binding protein (PTB) is involved in pre-mRNA splicing and internal-ribosomal-entry-site-dependent translation. The biochemical properties of various segments of PTB were analysed in order to understand the molecular basis of the PTB functions. The protein exists in oligomeric as well as monomeric form. The central part of PTB (amino acids 169-293) plays a major role in the oligomerization. PTB contains several RNA-binding motifs. Among them, the C-terminal part of PTB (amino acids 329-530) exhibited the strongest RNA-binding activity. The N-terminal part of PTB is responsible for the enhancement of RNA binding by HeLa cell cytoplasmic factor(s).

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalBiochemical Journal
Volume331
Issue number1
Publication statusPublished - 1998 Apr 1
Externally publishedYes

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Polypyrimidine Tract-Binding Protein
RNA
Amino Acids
Oligomerization
RNA Precursors
HeLa Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Oh, Y. L., Hahm, B., Kim, Y. K., Lee, H. K., Lee, J. W., Song, O. K., ... Jang, S. K. (1998). Determination of functional domains in polypyrimidine-tract-binding protein. Biochemical Journal, 331(1), 169-175.

Determination of functional domains in polypyrimidine-tract-binding protein. / Oh, Young L.; Hahm, Bumsuk; Kim, Yoon Ki; Lee, Hae K.; Lee, Joo W.; Song, Ok K.; Tsukiyama-Kohara, Kyoko; Kohara, Michinori; Nomoto, Akio; Jang, Sung K.

In: Biochemical Journal, Vol. 331, No. 1, 01.04.1998, p. 169-175.

Research output: Contribution to journalArticle

Oh, YL, Hahm, B, Kim, YK, Lee, HK, Lee, JW, Song, OK, Tsukiyama-Kohara, K, Kohara, M, Nomoto, A & Jang, SK 1998, 'Determination of functional domains in polypyrimidine-tract-binding protein', Biochemical Journal, vol. 331, no. 1, pp. 169-175.
Oh YL, Hahm B, Kim YK, Lee HK, Lee JW, Song OK et al. Determination of functional domains in polypyrimidine-tract-binding protein. Biochemical Journal. 1998 Apr 1;331(1):169-175.
Oh, Young L. ; Hahm, Bumsuk ; Kim, Yoon Ki ; Lee, Hae K. ; Lee, Joo W. ; Song, Ok K. ; Tsukiyama-Kohara, Kyoko ; Kohara, Michinori ; Nomoto, Akio ; Jang, Sung K. / Determination of functional domains in polypyrimidine-tract-binding protein. In: Biochemical Journal. 1998 ; Vol. 331, No. 1. pp. 169-175.
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