Determination of irsogladine using HPLC-ESI-MS/MS in human plasma: Application to bioequivalence study

Nguyen Huu Hoang, Nguyen Lan Huong, Sung Yong Hong, Je Won Park

Research output: Contribution to journalArticle

Abstract

A highly sensitive analytical tool for the fast quantification of irsogladine in human plasma was developed. Cleanup using a solid-phase extraction technique is a simple method for extracting both irsogladine and lamotrigine (internal standard) spiked into human plasma: 89.4 ± 2.4% for irsogladine and 85.9 ± 3.4% for lamotrigine. The resolvable separation of both analytes through reversed-phase high-performance liquid chromatography (HPLC) was carried out within 5 min. The HPLC-electrospray ionization (ESI)-tandem mass spectrometry (MS) method, which was operated in a selected reaction monitoring mode specific to the target analytes, was verified for use in the quantification of irsogladine. The inter- and intra-day precision (RSD) were <4% and their accuracies were between 85.9 to 89.8%. The calibration curve for irsogladine spiked into human plasma was linear over the range from 1 to 100 ng/mL; the limit of quantification was estimated to be 1.8 ng/mL. The established method was successfully applied for a bioequivalence study of irsogladine.

Original languageEnglish
Pages (from-to)1894-1898
Number of pages5
JournalLatin American Journal of Pharmacy
Volume35
Issue number8
Publication statusPublished - 2016 Jan 1

Fingerprint

irsogladine
Therapeutic Equivalency
Electrospray Ionization Mass Spectrometry
Tandem Mass Spectrometry
High Pressure Liquid Chromatography
Solid Phase Extraction
Reverse-Phase Chromatography
Calibration

Keywords

  • Bioequivalence study
  • HPLC-ESI-MS/MS
  • Human plasma
  • Irsogladine

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery

Cite this

Determination of irsogladine using HPLC-ESI-MS/MS in human plasma : Application to bioequivalence study. / Hoang, Nguyen Huu; Huong, Nguyen Lan; Hong, Sung Yong; Park, Je Won.

In: Latin American Journal of Pharmacy, Vol. 35, No. 8, 01.01.2016, p. 1894-1898.

Research output: Contribution to journalArticle

Hoang, Nguyen Huu ; Huong, Nguyen Lan ; Hong, Sung Yong ; Park, Je Won. / Determination of irsogladine using HPLC-ESI-MS/MS in human plasma : Application to bioequivalence study. In: Latin American Journal of Pharmacy. 2016 ; Vol. 35, No. 8. pp. 1894-1898.
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