Determination of red blood cell deformability using centrifugal force in a three-dimensional-printed mini-disk (3D-PMD)

Hyunjung Lim, Seung Min Back, Jeonghun Nam, Hyuk Choi

Research output: Contribution to journalArticle

Abstract

Measuring red blood cell (RBC) deformability has become important for clinical disease diagnostics. Various methods for measuring RBC deformability have been developed; however, they require costly and large instruments, long measuring time, and skilled personnel. In this study, we present a three-dimensional-printed mini-disk (3D-PMD) for measuring RBC deformability to overcome the previous limitations. For a miniaturized and low-cost setup, the 3D-PMD was fabricated by a 3D printing technique, which had not yet been used for fabricating a lab-on-a-compact disk (LOCD). Using a 3D printing technique, a multi-layered fluidic channel on the mini CD could be fabricated easily. During rotation by a spinning motor, the difference of the length of compressed RBCs in the fluidic channel was measured and analysed as compressibility indices (CIs) of normal and glutaraldehyde-treated hardened RBCs. The rotation speed and time were decided as 3000 rpm and 30 min, respectively, at which the difference of CI values between normal and hardened RBCs was largest (CInormal-CIhardened = 0.195).

Original languageEnglish
Article numbere0197619
JournalPLoS One
Volume13
Issue number5
DOIs
Publication statusPublished - 2018 May 1

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Polarization mode dispersion
Formability
Blood
erythrocytes
Erythrocytes
Cells
Fluidics
Compressibility
Printing
compressibility
Glutaral
Reference Values
Personnel
Costs and Cost Analysis
glutaraldehyde
spinning
human resources
Costs
methodology
Three Dimensional Printing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Determination of red blood cell deformability using centrifugal force in a three-dimensional-printed mini-disk (3D-PMD). / Lim, Hyunjung; Back, Seung Min; Nam, Jeonghun; Choi, Hyuk.

In: PLoS One, Vol. 13, No. 5, e0197619, 01.05.2018.

Research output: Contribution to journalArticle

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