TY - JOUR
T1 - Determination of the core promoter regions of the Saccharomyces cerevisiae RPS3 gene
AU - Joo, Yoo Jin
AU - Kim, Jin ha
AU - Baek, Joung Hee
AU - Seong, Ki Moon
AU - Lee, Jae Yung
AU - Kim, Joon
N1 - Funding Information:
This work was supported in part by Proteomics grants FPR05C2-390 and KRF-2008-C00787 .
PY - 2009/11
Y1 - 2009/11
N2 - Ribosomal protein genes (RPG), which are scattered throughout the genomes of all eukaryotes, are subjected to coordinated expression. In yeast, the expression of RPGs is highly regulated, mainly at the transcriptional level. Recent research has found that many ribosomal proteins (RPs) function in multiple processes in addition to protein synthesis. Therefore, detailed knowledge of promoter architecture as well as gene regulation is important in understanding the multiple cellular processes mediated by RPGs. In this study, we investigated the functional architecture of the yeast RPS3 promoter and identified many putative cis-elements. Using β-galactosidase reporter analysis and EMSA, the core promoter of RPS3 containing UAS rpg and T-rich regions was corroborated. Moreover, the promoter occupancy of RPS3 by three transcription factors was confirmed. Taken together, our results further the current understanding of the promoter architecture and trans-elements of the Saccharomyces cerevisiae RPS3 gene.
AB - Ribosomal protein genes (RPG), which are scattered throughout the genomes of all eukaryotes, are subjected to coordinated expression. In yeast, the expression of RPGs is highly regulated, mainly at the transcriptional level. Recent research has found that many ribosomal proteins (RPs) function in multiple processes in addition to protein synthesis. Therefore, detailed knowledge of promoter architecture as well as gene regulation is important in understanding the multiple cellular processes mediated by RPGs. In this study, we investigated the functional architecture of the yeast RPS3 promoter and identified many putative cis-elements. Using β-galactosidase reporter analysis and EMSA, the core promoter of RPS3 containing UAS rpg and T-rich regions was corroborated. Moreover, the promoter occupancy of RPS3 by three transcription factors was confirmed. Taken together, our results further the current understanding of the promoter architecture and trans-elements of the Saccharomyces cerevisiae RPS3 gene.
KW - Rap1p
KW - Ribosomal protein gene
KW - T-rich region
KW - Transcription factor
KW - UAS
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U2 - 10.1016/j.bbagrm.2009.10.002
DO - 10.1016/j.bbagrm.2009.10.002
M3 - Article
C2 - 19853675
AN - SCOPUS:77949316590
VL - 1789
SP - 741
EP - 750
JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
JF - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
SN - 1874-9399
IS - 11-12
ER -