Determination of tocopherol contents in refined edible oils using an HPLC method

Jiang Ning Hu, Xue Mei Zhu, Prakash Adhikari, Dan Li, In-Hwan Kim, Ki Teak Lee

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A high-performance liquid chromatography method was applied to determine the contents of tocopherols in edible oils using a LiChrosorb DIOL HPLC column and hexane fortified with 0.1% acetic acid in an isocratic mode. The validation of the method included tests for linearity, sensitivity, accuracy, precision, and recovery. All calibration curves showed good linear regression (r 2>0.9995) within the tested ranges. The established method offered good precision and accuracy with overall intra-day and inter-day variations of 0.94∼4.27 and 1.77∼4.88%, respectively. The tocopherol recoveries ranged from 91.44∼108.90%. Subsequently, the method was successfully applied to qualitatively and quantitatively determine the total contents of α, γ, and δ-tocopherols in 12 selected refined edible oils, showing a range of 0.92 to 188.71 mg/100 g.

Original languageEnglish
Pages (from-to)260-264
Number of pages5
JournalJournal of Food Science and Nutrition
Volume14
Issue number3
DOIs
Publication statusPublished - 2009 Dec 1

Fingerprint

Tocopherols
tocopherols
Oils
high performance liquid chromatography
High Pressure Liquid Chromatography
oils
Hexanes
methodology
Acetic Acid
hexane
Calibration
acetic acid
Linear Models
calibration
testing

Keywords

  • HPLC separation
  • Refined edible oils
  • Tocopherols

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

Cite this

Determination of tocopherol contents in refined edible oils using an HPLC method. / Hu, Jiang Ning; Zhu, Xue Mei; Adhikari, Prakash; Li, Dan; Kim, In-Hwan; Lee, Ki Teak.

In: Journal of Food Science and Nutrition, Vol. 14, No. 3, 01.12.2009, p. 260-264.

Research output: Contribution to journalArticle

Hu, Jiang Ning ; Zhu, Xue Mei ; Adhikari, Prakash ; Li, Dan ; Kim, In-Hwan ; Lee, Ki Teak. / Determination of tocopherol contents in refined edible oils using an HPLC method. In: Journal of Food Science and Nutrition. 2009 ; Vol. 14, No. 3. pp. 260-264.
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