Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration

Jaeyeon Lee, Se Hwan Lee, Byung Soo Kim, Young Sam Cho, Yongdoo Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background:: Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures. Methods:: In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bio-active peptides and tyramine-conjugated hyaluronic acids for fast gelation. Results:: Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bio-ink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed > 90% viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides: BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features. Conclusion:: We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine.

Original languageEnglish
Pages (from-to)761-769
Number of pages9
JournalTissue Engineering and Regenerative Medicine
Volume15
Issue number6
DOIs
Publication statusPublished - 2018 Dec 1

Fingerprint

Hyaluronic acid
Tissue regeneration
Ink
Hyaluronic Acid
Regeneration
Bioprinting
Peptides
Cues
Bone Morphogenetic Protein 7
Printing
Regenerative Medicine
Gelation
Substance P
Stem cells
Cell Differentiation
Cells
Tyramine
Osteopontin
Hydrogels
Fibroblasts

Keywords

  • Bio-ink
  • Bioprinting
  • Hyaluronic acid
  • Hydrogel
  • Tissue engineering

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration. / Lee, Jaeyeon; Lee, Se Hwan; Kim, Byung Soo; Cho, Young Sam; Park, Yongdoo.

In: Tissue Engineering and Regenerative Medicine, Vol. 15, No. 6, 01.12.2018, p. 761-769.

Research output: Contribution to journalArticle

@article{e79265a772714d908cf887e350fc9124,
title = "Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration",
abstract = "Background:: Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures. Methods:: In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bio-active peptides and tyramine-conjugated hyaluronic acids for fast gelation. Results:: Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bio-ink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed > 90{\%} viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides: BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features. Conclusion:: We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine.",
keywords = "Bio-ink, Bioprinting, Hyaluronic acid, Hydrogel, Tissue engineering",
author = "Jaeyeon Lee and Lee, {Se Hwan} and Kim, {Byung Soo} and Cho, {Young Sam} and Yongdoo Park",
year = "2018",
month = "12",
day = "1",
doi = "10.1007/s13770-018-0144-8",
language = "English",
volume = "15",
pages = "761--769",
journal = "Tissue Engineering and Regenerative Medicine",
issn = "1738-2696",
publisher = "Springer Science + Business Media",
number = "6",

}

TY - JOUR

T1 - Development and Evaluation of Hyaluronic Acid-Based Hybrid Bio-Ink for Tissue Regeneration

AU - Lee, Jaeyeon

AU - Lee, Se Hwan

AU - Kim, Byung Soo

AU - Cho, Young Sam

AU - Park, Yongdoo

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Background:: Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures. Methods:: In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bio-active peptides and tyramine-conjugated hyaluronic acids for fast gelation. Results:: Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bio-ink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed > 90% viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides: BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features. Conclusion:: We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine.

AB - Background:: Bioprinting has recently appeared as a powerful tool for building complex tissue and organ structures. However, the application of bioprinting to regenerative medicine has limitations, due to the restricted choices of bio-ink for cytocompatible cell encapsulation and the integrity of the fabricated structures. Methods:: In this study, we developed hybrid bio-inks based on acrylated hyaluronic acid (HA) for immobilizing bio-active peptides and tyramine-conjugated hyaluronic acids for fast gelation. Results:: Conventional acrylated HA-based hydrogels have a gelation time of more than 30 min, whereas hybrid bio-ink has been rapidly gelated within 200 s. Fibroblast cells cultured in this hybrid bio-ink up to 7 days showed > 90% viability. As a guidance cue for stem cell differentiation, we immobilized four different bio-active peptides: BMP-7-derived peptides (BMP-7D) and osteopontin for osteogenesis, and substance-P (SP) and Ac-SDKP (SDKP) for angiogenesis. Mesenchymal stem cells cultured in these hybrid bio-inks showed the highest angiogenic and osteogenic activity cultured in bio-ink immobilized with a SP or BMP-7D peptide. This bio-ink was loaded in a three-dimensional (3D) bioprinting device showing reproducible printing features. Conclusion:: We have developed bio-inks that combine biochemical and mechanical cues. Biochemical cues were able to regulate differentiation of cells, and mechanical cues enabled printing structuring. This multi-functional bio-ink can be used for complex tissue engineering and regenerative medicine.

KW - Bio-ink

KW - Bioprinting

KW - Hyaluronic acid

KW - Hydrogel

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85056992784&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056992784&partnerID=8YFLogxK

U2 - 10.1007/s13770-018-0144-8

DO - 10.1007/s13770-018-0144-8

M3 - Article

AN - SCOPUS:85056992784

VL - 15

SP - 761

EP - 769

JO - Tissue Engineering and Regenerative Medicine

JF - Tissue Engineering and Regenerative Medicine

SN - 1738-2696

IS - 6

ER -