TY - JOUR
T1 - Development and method validation of analysis of urushiol in sumac and food ingredients in Korea
AU - Seo, Dongwon
AU - Kim, Kijin
AU - Choi, Hee Don
AU - Yoo, Miyoung
AU - Ha, Jaeho
AU - Lee, Kwang Won
N1 - Funding Information:
Received June 19, 2017. Accepted by AP August 7, 2017. 1Corresponding author’s e-mail: kwangwon@korea.ac.kr This research was supported by the Main Research Program (E0143033673) of the Korea Food Research Institute, funded by the Korean Ministry of Science and ICT. DOI: https://doi.org/10.5740/jaoacint.17-0252
Publisher Copyright:
© 2018 AOAC International. All Rights Reserved.
PY - 2018
Y1 - 2018
N2 - This study developed an analytical method to determine the urushiol content in sap and several foods. The full process for urushiol analysis consists of extraction, trimethylsilyl silylation, analysis, and identification via GC-MS, with each step optimized to attain the required accuracy and precision. Urushiol was separated from sap via liquid–liquid extraction and was derivatized via silylation. The components were analyzed using a polar capillary column and identified using GC-MS. The deviations of relative retention times and retention time windows were within 0.001 and 0.02 min, which satisfied the criteria of 0.06 and 0.03 min, respectively. The response of the urushiol standards tested was found to be linear in the investigated concentration range, with a correlation coefficient of 0.998. The LODs were between 1.74 and 2.67 µg/mL.
AB - This study developed an analytical method to determine the urushiol content in sap and several foods. The full process for urushiol analysis consists of extraction, trimethylsilyl silylation, analysis, and identification via GC-MS, with each step optimized to attain the required accuracy and precision. Urushiol was separated from sap via liquid–liquid extraction and was derivatized via silylation. The components were analyzed using a polar capillary column and identified using GC-MS. The deviations of relative retention times and retention time windows were within 0.001 and 0.02 min, which satisfied the criteria of 0.06 and 0.03 min, respectively. The response of the urushiol standards tested was found to be linear in the investigated concentration range, with a correlation coefficient of 0.998. The LODs were between 1.74 and 2.67 µg/mL.
UR - http://www.scopus.com/inward/record.url?scp=85058382072&partnerID=8YFLogxK
U2 - 10.5740/jaoacint.17-0252
DO - 10.5740/jaoacint.17-0252
M3 - Article
C2 - 28958219
AN - SCOPUS:85058382072
VL - 101
SP - 793
EP - 797
JO - Journal of AOAC International
JF - Journal of AOAC International
SN - 1060-3271
IS - 3
ER -