Abstract
This study developed an analytical method to determine the urushiol content in sap and several foods. The full process for urushiol analysis consists of extraction, trimethylsilyl silylation, analysis, and identification via GC-MS, with each step optimized to attain the required accuracy and precision. Urushiol was separated from sap via liquid–liquid extraction and was derivatized via silylation. The components were analyzed using a polar capillary column and identified using GC-MS. The deviations of relative retention times and retention time windows were within 0.001 and 0.02 min, which satisfied the criteria of 0.06 and 0.03 min, respectively. The response of the urushiol standards tested was found to be linear in the investigated concentration range, with a correlation coefficient of 0.998. The LODs were between 1.74 and 2.67 µg/mL.
| Original language | English |
|---|---|
| Pages (from-to) | 793-797 |
| Number of pages | 5 |
| Journal | Journal of AOAC International |
| Volume | 101 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2018 Jan 1 |
Fingerprint
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science
- Environmental Chemistry
- Agronomy and Crop Science
- Pharmacology
Cite this
Development and method validation of analysis of urushiol in sumac and food ingredients in Korea. / Seo, Dongwon; Kim, Kijin; Choi, Hee Don; Yoo, Miyoung; Ha, Jaeho; Lee, Kwang Won.
In: Journal of AOAC International, Vol. 101, No. 3, 01.01.2018, p. 793-797.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development and method validation of analysis of urushiol in sumac and food ingredients in Korea
AU - Seo, Dongwon
AU - Kim, Kijin
AU - Choi, Hee Don
AU - Yoo, Miyoung
AU - Ha, Jaeho
AU - Lee, Kwang Won
PY - 2018/1/1
Y1 - 2018/1/1
N2 - This study developed an analytical method to determine the urushiol content in sap and several foods. The full process for urushiol analysis consists of extraction, trimethylsilyl silylation, analysis, and identification via GC-MS, with each step optimized to attain the required accuracy and precision. Urushiol was separated from sap via liquid–liquid extraction and was derivatized via silylation. The components were analyzed using a polar capillary column and identified using GC-MS. The deviations of relative retention times and retention time windows were within 0.001 and 0.02 min, which satisfied the criteria of 0.06 and 0.03 min, respectively. The response of the urushiol standards tested was found to be linear in the investigated concentration range, with a correlation coefficient of 0.998. The LODs were between 1.74 and 2.67 µg/mL.
AB - This study developed an analytical method to determine the urushiol content in sap and several foods. The full process for urushiol analysis consists of extraction, trimethylsilyl silylation, analysis, and identification via GC-MS, with each step optimized to attain the required accuracy and precision. Urushiol was separated from sap via liquid–liquid extraction and was derivatized via silylation. The components were analyzed using a polar capillary column and identified using GC-MS. The deviations of relative retention times and retention time windows were within 0.001 and 0.02 min, which satisfied the criteria of 0.06 and 0.03 min, respectively. The response of the urushiol standards tested was found to be linear in the investigated concentration range, with a correlation coefficient of 0.998. The LODs were between 1.74 and 2.67 µg/mL.
UR - http://www.scopus.com/inward/record.url?scp=85058382072&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85058382072&partnerID=8YFLogxK
U2 - 10.5740/jaoacint.17-0252
DO - 10.5740/jaoacint.17-0252
M3 - Article
C2 - 28958219
AN - SCOPUS:85058382072
VL - 101
SP - 793
EP - 797
JO - Journal of AOAC International
JF - Journal of AOAC International
SN - 1060-3271
IS - 3
ER -