Development of a Cheap and Portable Sensing System for Electrochemical Genotyping

Sungbo Cho, Nakseon Seong, James Jungho Pak

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

This paper reports a cheap and portable electrochemical sensing system, which is based on AT90S8535 one-chip micro-controller, for electrochemical genotyping using a DNA chip. The proposed electrochemical sensing system consists of a signal input part including 555-timer based circuit, a signal detection part including T-feedback amplifier based circuit, a signal control part including AT90S8535, and an output display part. Au was used as electrode material, 2-mercaptoethanol was used as self-assembled monolayer for linking DNA to Au electrode, and methylene blue was used as an indicator that can be selectively bound to double stranded DNAs. By this sensing system, the measured reductive peak voltage of double stranded DNAs (148mV) was larger by approximately 74 mV than that of the single stranded DNAs (74mV). Through the analysis of these values measured from DNA modified electrodes, it is possible to distinguish double stranded DNAs from single stranded DNAs. Thus, the result of genotyping is able to be displayed onto LCD or to be transmitted to a computer in just several seconds.

Original languageEnglish
Title of host publicationProceedings of IEEE Sensors
Pages233-236
Number of pages4
Volume1
Edition1
Publication statusPublished - 2002 Dec 1
EventFirst IEEE International Conference on Sensors - IEEE Sensors 2002 - Orlando, FL, United States
Duration: 2002 Jun 122002 Jun 14

Other

OtherFirst IEEE International Conference on Sensors - IEEE Sensors 2002
CountryUnited States
CityOrlando, FL
Period02/6/1202/6/14

Fingerprint

DNA
Electrodes
Feedback amplifiers
Networks (circuits)
Signal detection
Self assembled monolayers
Liquid crystal displays
Display devices
Controllers
Electric potential

Keywords

  • AT90S8535 one-chip micro-controller
  • Double stranded DNA
  • Methylene blue

ASJC Scopus subject areas

  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

Cite this

Cho, S., Seong, N., & Pak, J. J. (2002). Development of a Cheap and Portable Sensing System for Electrochemical Genotyping. In Proceedings of IEEE Sensors (1 ed., Vol. 1, pp. 233-236)

Development of a Cheap and Portable Sensing System for Electrochemical Genotyping. / Cho, Sungbo; Seong, Nakseon; Pak, James Jungho.

Proceedings of IEEE Sensors. Vol. 1 1. ed. 2002. p. 233-236.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cho, S, Seong, N & Pak, JJ 2002, Development of a Cheap and Portable Sensing System for Electrochemical Genotyping. in Proceedings of IEEE Sensors. 1 edn, vol. 1, pp. 233-236, First IEEE International Conference on Sensors - IEEE Sensors 2002, Orlando, FL, United States, 02/6/12.
Cho S, Seong N, Pak JJ. Development of a Cheap and Portable Sensing System for Electrochemical Genotyping. In Proceedings of IEEE Sensors. 1 ed. Vol. 1. 2002. p. 233-236
Cho, Sungbo ; Seong, Nakseon ; Pak, James Jungho. / Development of a Cheap and Portable Sensing System for Electrochemical Genotyping. Proceedings of IEEE Sensors. Vol. 1 1. ed. 2002. pp. 233-236
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