Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes

Honggu Chun, Patty J. Dennis, Erin R. Ferguson Welch, Jean Pierre Alarie, James W. Jorgenson, J. Michael Ramsey

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500. nM and 0.55. nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated.

Original languageEnglish
JournalJournal of Chromatography A
DOIs
Publication statusAccepted/In press - 2017

Fingerprint

Electrodes
Gels
rhodamine B
Glucosamine
Electric potential
Lasers
Microfluidics
Heat generation
Frequency modulation
Viscosity
Limit of Detection
Potassium
Hot Temperature
Electric fields
Light

Keywords

  • Label-free detection
  • Microfluidics
  • Photothermal absorbance detection
  • Polyelectrolytic gel electrode

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes. / Chun, Honggu; Dennis, Patty J.; Ferguson Welch, Erin R.; Alarie, Jean Pierre; Jorgenson, James W.; Ramsey, J. Michael.

In: Journal of Chromatography A, 2017.

Research output: Contribution to journalArticle

Chun, Honggu ; Dennis, Patty J. ; Ferguson Welch, Erin R. ; Alarie, Jean Pierre ; Jorgenson, James W. ; Ramsey, J. Michael. / Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes. In: Journal of Chromatography A. 2017.
@article{725b2bbd8528465f8cc2325684821ed2,
title = "Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes",
abstract = "The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500. nM and 0.55. nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated.",
keywords = "Label-free detection, Microfluidics, Photothermal absorbance detection, Polyelectrolytic gel electrode",
author = "Honggu Chun and Dennis, {Patty J.} and {Ferguson Welch}, {Erin R.} and Alarie, {Jean Pierre} and Jorgenson, {James W.} and Ramsey, {J. Michael}",
year = "2017",
doi = "10.1016/j.chroma.2017.06.053",
language = "English",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",

}

TY - JOUR

T1 - Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel electrodes

AU - Chun, Honggu

AU - Dennis, Patty J.

AU - Ferguson Welch, Erin R.

AU - Alarie, Jean Pierre

AU - Jorgenson, James W.

AU - Ramsey, J. Michael

PY - 2017

Y1 - 2017

N2 - The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500. nM and 0.55. nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated.

AB - The development and application of polyelectrolytic gel electrodes (PGEs) for a microfluidic photothermal absorbance detection system is described. The PGEs are used to measure changes in conductivity based on heat generation by analytes absorbing light and changing the solution viscosity. The PGEs are suitable for direct contact conductivity measurements since they do not degrade with exposure to high electric fields. Both a 2-electrode system with DC voltages and a 3-electrode system with AC voltages were investigated. Experimental factors including excitation voltage, excitation frequency, laser modulation frequency, laser power, and path length were tested. The limits of detection for the 3-electrode and 2-electrode systems are 500. nM and 0.55. nM for DABSYL-tagged glucosamine, respectively. In addition, an electrokinetic separation of a potassium, DABSYL-tagged glucosamine, Rhodamine 6G, and Rhodamine B mixture was demonstrated.

KW - Label-free detection

KW - Microfluidics

KW - Photothermal absorbance detection

KW - Polyelectrolytic gel electrode

UR - http://www.scopus.com/inward/record.url?scp=85021273989&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85021273989&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2017.06.053

DO - 10.1016/j.chroma.2017.06.053

M3 - Article

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

ER -