Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval

Edward Kang, Yoon Young Choi, Yesl Jun, Bong Geun Chung, Sang Hoon Lee

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

We have developed a multi-layer, microfluidic array platform containing concave microwells and flat cell culture chambers to culture embryonic stem (ES) cells and regulate uniform-sized embryoid body (EB) formation. The main advantage of this platform was that EBs cultured within the concave microwells of a bottom layer were automatically replated into flat cell culture chambers of a top layer, following inversion of the multi-layer microfluidic array platform. This allowed EB formation and EB replating to be controlled simultaneously inside a single microfluidic device without pipette-based manual cell retrieval, a drawback of previous EB culture methods.

Original languageEnglish
Pages (from-to)2651-2654
Number of pages4
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume10
Issue number20
DOIs
Publication statusPublished - 2010 Oct 21

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Embryoid Bodies
Microfluidics
Cell culture
Lab-On-A-Chip Devices
Inversion layers
Cell Culture Techniques
Stem cells
Embryonic Stem Cells

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval. / Kang, Edward; Choi, Yoon Young; Jun, Yesl; Chung, Bong Geun; Lee, Sang Hoon.

In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 10, No. 20, 21.10.2010, p. 2651-2654.

Research output: Contribution to journalArticle

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