Development of a multiplex loop-mediated isothermal amplification assay for diagnosis of plasmodium spp., plasmodium falciparum and plasmodium vivax

Woong Sik Jang, Da Hye Lim, Younglan Choe, Hyunseul Jee, Kyung Chul Moon, Chaewon Kim, Minkyeong Choi, In Su Park, Chae Seung Lim

Research output: Contribution to journalArticlepeer-review

Abstract

Malaria, caused by the parasite Plasmodium and transmitted by mosquitoes, is an epidemic that mainly occurs in tropical and subtropical regions. As treatments differ across species of malarial parasites, there is a need to develop rapid diagnostic methods to differentiate malarial species. Herein, we developed a multiplex malaria Pan/Pf/Pv/actin beta loop-mediated isothermal amplification (LAMP) to diagnose Plasmodium spp., P. falciparum, and P. vivax, as well as the internal control (IC), within 40 min. The detection limits of the multiplex malaria Pan/Pf/Pv/IC LAMP were 1 × 102, 1 × 102, 1 × 102, and 1 × 103 copies/µL for four vectors, including the 18S rRNA gene (Plasmodium spp.), lactate dehydrogenase gene (P. falciparum), 16S rRNA gene (P. vivax), and human actin beta gene (IC), respectively. The performance of the LAMP assay was compared and evaluated by evaluating 208 clinical samples (118 positive and 90 negative samples) with the commercial RealStar® Malaria S&T PCR Kit 1.0. The developed multiplex malaria Pan/Pf/Pv/IC LAMP assay showed comparable sensitivity (100%) and specificity (100%) with the commercial RealStar® Malaria S&T PCR Kit 1.0 (100%). These results suggest that the multiplex malaria Pan/Pf/Pv/IC LAMP could be used as a point-of-care molecular diagnostic test for malaria.

Original languageEnglish
Article number1950
JournalDiagnostics
Volume11
Issue number11
DOIs
Publication statusPublished - 2021 Nov

Keywords

  • LAMP
  • Multiplex LAMP
  • Plasmodium falciparum
  • Plasmodium spp
  • Plasmodium vivax

ASJC Scopus subject areas

  • Clinical Biochemistry

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