Development of a novel screening system for allatostatin receptor agonists in search of new candidate insect growth regulators

Joonho Na, Wonju Kim, Bong June Yoon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Allatostatins (ASTs) are insect neuropeptide hormones that regulate diverse physiological functions, including feeding, growth and development, and reproduction. Therefore, regulation of allatostatin receptor (AstR) activity can be an effective tool for controlling insect growth and proliferation. Here, we describe a novel screening system using a mammalian cell line in which AstR is ectopically expressed, combined with fluorescence-based measurements of the membrane potential. HEK293T cells that do not express cognate receptors for AST became responsive to AST upon transfection with AstR. The response of the membrane potential to AST could be reliably detected by measuring the fluorescence of DiBAC4(3), a voltage-sensitive dye. We also discovered that overexpressing GIRK1/2 in this cell line could augment the magnitude of hyperpolarization by AST. Our screening system produces a fast and reliable readout for the efficient screening of AstR agonists.

Original languageEnglish
Pages (from-to)1439-1446
Number of pages8
JournalBiotechnology letters
Volume36
Issue number7
DOIs
Publication statusPublished - 2014 Jun

Keywords

  • Allatostatin
  • Allatostatin agonist
  • GIRK1/2
  • Insect growth regulators
  • Neuropeptide hormone
  • Pest control
  • Screening system

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Development of a novel screening system for allatostatin receptor agonists in search of new candidate insect growth regulators'. Together they form a unique fingerprint.

  • Cite this