Development of a novel screening system for allatostatin receptor agonists in search of new candidate insect growth regulators

Joonho Na, Wonju Kim, Bongjune Yoon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Allatostatins (ASTs) are insect neuropeptide hormones that regulate diverse physiological functions, including feeding, growth and development, and reproduction. Therefore, regulation of allatostatin receptor (AstR) activity can be an effective tool for controlling insect growth and proliferation. Here, we describe a novel screening system using a mammalian cell line in which AstR is ectopically expressed, combined with fluorescence-based measurements of the membrane potential. HEK293T cells that do not express cognate receptors for AST became responsive to AST upon transfection with AstR. The response of the membrane potential to AST could be reliably detected by measuring the fluorescence of DiBAC4(3), a voltage-sensitive dye. We also discovered that overexpressing GIRK1/2 in this cell line could augment the magnitude of hyperpolarization by AST. Our screening system produces a fast and reliable readout for the efficient screening of AstR agonists.

Original languageEnglish
Pages (from-to)1439-1446
Number of pages8
JournalBiotechnology Letters
Volume36
Issue number7
DOIs
Publication statusPublished - 2014 Jan 1

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Juvenile Hormones
Membrane Potentials
Insect Hormones
Fluorescence
allatostatin
Cell Line
Neuropeptides
Growth and Development
Reproduction
Transfection
Insects
Coloring Agents

ASJC Scopus subject areas

  • Biotechnology

Cite this

Development of a novel screening system for allatostatin receptor agonists in search of new candidate insect growth regulators. / Na, Joonho; Kim, Wonju; Yoon, Bongjune.

In: Biotechnology Letters, Vol. 36, No. 7, 01.01.2014, p. 1439-1446.

Research output: Contribution to journalArticle

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