Development of a pH sensitive nanocarrier using calcium phosphate coated gold nanoparticles as a platform for a potential theranostic material

Eui Joon Cha, In Cheol Sun, Sang Cheon Lee, Kwang Meyung Kim, Ick Chan Kwon, Cheol Hee Ahn

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Methoxy-poly(ethylene glycol) (mPEG)-Asp-Cys was synthesized by ring opening polymerization, and its characterization by 1H nuclear magnetic resonance (NMR) showed that the copolymer has 18 aspartic acid units and 0.95 cysteine units on average, with the molecular weight and distribution of 13.1 k and 1.15. PEGylated AuNP with doxorubicin containing CaP layers was prepared to have the size of 79.8±18.7 nm by light scattering measurement method, with the CaP thickness around 27 nm. PEGylated Dox-AuNP@CaP was stable in the physiological conditions for several weeks in the presence of glutathione. Loading amount and efficiency of doxorubicin into the particles were 1.3% and 27.9%, respectively. The loading amount was 1.3%, lower than common organic self-assembled carriers, because of the relatively high atomic mass of gold. In vitro release experiments were performed in two different environments: extracellular fluid (pH 7.4) and intracellular lyosomal fluid (pH 4.5). The amount of released doxorubicin from PEGylated Dox-AuNP, without CaP layer, exceeds 60% in 2 h. In contrast, PEGylated Dox-AuNP@CaP effectively limited the doxorubicin release in the extracellular condition to 13% after 12 h, and changing the media to the intracellular lysosomal condition resulted in a triggered release of doxorubicin from 7% to 78% in an additional 4 h. Cell viability of PEGylated Dox-AuNP@CaP and PEGylated AuNP@CaP were estimated with HeLa cells by MTT assay. Cell viability of PEGylated Dox-AuNP@CaP came down to 19% after 24 h incubation, while the PEGylated AuNP@CaP particle itself showed slight cytotoxicity with the same conditions. The prepared PEGylated Dox-AuNP@CaP worked as a pH- and ion concentration-sensitive drug-delivery system as well as theranostic nanoparticles for CT imaging and cancer treatment.

Original languageEnglish
Pages (from-to)319-326
Number of pages8
JournalMacromolecular Research
Volume20
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1
Externally publishedYes

Fingerprint

Calcium phosphate
Gold
Doxorubicin
Cells
Nanoparticles
Oncology
Fluids
Ring opening polymerization
Cytotoxicity
Light scattering
Polyethylene glycols
Assays
Copolymers
Molecular weight
Nuclear magnetic resonance
Imaging techniques
Acids
Ions
Aspartic Acid
Experiments

Keywords

  • Calcium phosphate
  • Computed tomography (CT)
  • Gold nanoparticle
  • PH sensitive
  • Theranostics

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics
  • Chemical Engineering(all)

Cite this

Development of a pH sensitive nanocarrier using calcium phosphate coated gold nanoparticles as a platform for a potential theranostic material. / Cha, Eui Joon; Sun, In Cheol; Lee, Sang Cheon; Kim, Kwang Meyung; Kwon, Ick Chan; Ahn, Cheol Hee.

In: Macromolecular Research, Vol. 20, No. 3, 01.03.2012, p. 319-326.

Research output: Contribution to journalArticle

Cha, Eui Joon ; Sun, In Cheol ; Lee, Sang Cheon ; Kim, Kwang Meyung ; Kwon, Ick Chan ; Ahn, Cheol Hee. / Development of a pH sensitive nanocarrier using calcium phosphate coated gold nanoparticles as a platform for a potential theranostic material. In: Macromolecular Research. 2012 ; Vol. 20, No. 3. pp. 319-326.
@article{a6bb679249a64b25bbf0b785b9fddfa9,
title = "Development of a pH sensitive nanocarrier using calcium phosphate coated gold nanoparticles as a platform for a potential theranostic material",
abstract = "Methoxy-poly(ethylene glycol) (mPEG)-Asp-Cys was synthesized by ring opening polymerization, and its characterization by 1H nuclear magnetic resonance (NMR) showed that the copolymer has 18 aspartic acid units and 0.95 cysteine units on average, with the molecular weight and distribution of 13.1 k and 1.15. PEGylated AuNP with doxorubicin containing CaP layers was prepared to have the size of 79.8±18.7 nm by light scattering measurement method, with the CaP thickness around 27 nm. PEGylated Dox-AuNP@CaP was stable in the physiological conditions for several weeks in the presence of glutathione. Loading amount and efficiency of doxorubicin into the particles were 1.3{\%} and 27.9{\%}, respectively. The loading amount was 1.3{\%}, lower than common organic self-assembled carriers, because of the relatively high atomic mass of gold. In vitro release experiments were performed in two different environments: extracellular fluid (pH 7.4) and intracellular lyosomal fluid (pH 4.5). The amount of released doxorubicin from PEGylated Dox-AuNP, without CaP layer, exceeds 60{\%} in 2 h. In contrast, PEGylated Dox-AuNP@CaP effectively limited the doxorubicin release in the extracellular condition to 13{\%} after 12 h, and changing the media to the intracellular lysosomal condition resulted in a triggered release of doxorubicin from 7{\%} to 78{\%} in an additional 4 h. Cell viability of PEGylated Dox-AuNP@CaP and PEGylated AuNP@CaP were estimated with HeLa cells by MTT assay. Cell viability of PEGylated Dox-AuNP@CaP came down to 19{\%} after 24 h incubation, while the PEGylated AuNP@CaP particle itself showed slight cytotoxicity with the same conditions. The prepared PEGylated Dox-AuNP@CaP worked as a pH- and ion concentration-sensitive drug-delivery system as well as theranostic nanoparticles for CT imaging and cancer treatment.",
keywords = "Calcium phosphate, Computed tomography (CT), Gold nanoparticle, PH sensitive, Theranostics",
author = "Cha, {Eui Joon} and Sun, {In Cheol} and Lee, {Sang Cheon} and Kim, {Kwang Meyung} and Kwon, {Ick Chan} and Ahn, {Cheol Hee}",
year = "2012",
month = "3",
day = "1",
doi = "10.1007/s13233-012-0061-y",
language = "English",
volume = "20",
pages = "319--326",
journal = "Macromolecular Research",
issn = "1598-5032",
publisher = "Polymer Society of Korea",
number = "3",

}

TY - JOUR

T1 - Development of a pH sensitive nanocarrier using calcium phosphate coated gold nanoparticles as a platform for a potential theranostic material

AU - Cha, Eui Joon

AU - Sun, In Cheol

AU - Lee, Sang Cheon

AU - Kim, Kwang Meyung

AU - Kwon, Ick Chan

AU - Ahn, Cheol Hee

PY - 2012/3/1

Y1 - 2012/3/1

N2 - Methoxy-poly(ethylene glycol) (mPEG)-Asp-Cys was synthesized by ring opening polymerization, and its characterization by 1H nuclear magnetic resonance (NMR) showed that the copolymer has 18 aspartic acid units and 0.95 cysteine units on average, with the molecular weight and distribution of 13.1 k and 1.15. PEGylated AuNP with doxorubicin containing CaP layers was prepared to have the size of 79.8±18.7 nm by light scattering measurement method, with the CaP thickness around 27 nm. PEGylated Dox-AuNP@CaP was stable in the physiological conditions for several weeks in the presence of glutathione. Loading amount and efficiency of doxorubicin into the particles were 1.3% and 27.9%, respectively. The loading amount was 1.3%, lower than common organic self-assembled carriers, because of the relatively high atomic mass of gold. In vitro release experiments were performed in two different environments: extracellular fluid (pH 7.4) and intracellular lyosomal fluid (pH 4.5). The amount of released doxorubicin from PEGylated Dox-AuNP, without CaP layer, exceeds 60% in 2 h. In contrast, PEGylated Dox-AuNP@CaP effectively limited the doxorubicin release in the extracellular condition to 13% after 12 h, and changing the media to the intracellular lysosomal condition resulted in a triggered release of doxorubicin from 7% to 78% in an additional 4 h. Cell viability of PEGylated Dox-AuNP@CaP and PEGylated AuNP@CaP were estimated with HeLa cells by MTT assay. Cell viability of PEGylated Dox-AuNP@CaP came down to 19% after 24 h incubation, while the PEGylated AuNP@CaP particle itself showed slight cytotoxicity with the same conditions. The prepared PEGylated Dox-AuNP@CaP worked as a pH- and ion concentration-sensitive drug-delivery system as well as theranostic nanoparticles for CT imaging and cancer treatment.

AB - Methoxy-poly(ethylene glycol) (mPEG)-Asp-Cys was synthesized by ring opening polymerization, and its characterization by 1H nuclear magnetic resonance (NMR) showed that the copolymer has 18 aspartic acid units and 0.95 cysteine units on average, with the molecular weight and distribution of 13.1 k and 1.15. PEGylated AuNP with doxorubicin containing CaP layers was prepared to have the size of 79.8±18.7 nm by light scattering measurement method, with the CaP thickness around 27 nm. PEGylated Dox-AuNP@CaP was stable in the physiological conditions for several weeks in the presence of glutathione. Loading amount and efficiency of doxorubicin into the particles were 1.3% and 27.9%, respectively. The loading amount was 1.3%, lower than common organic self-assembled carriers, because of the relatively high atomic mass of gold. In vitro release experiments were performed in two different environments: extracellular fluid (pH 7.4) and intracellular lyosomal fluid (pH 4.5). The amount of released doxorubicin from PEGylated Dox-AuNP, without CaP layer, exceeds 60% in 2 h. In contrast, PEGylated Dox-AuNP@CaP effectively limited the doxorubicin release in the extracellular condition to 13% after 12 h, and changing the media to the intracellular lysosomal condition resulted in a triggered release of doxorubicin from 7% to 78% in an additional 4 h. Cell viability of PEGylated Dox-AuNP@CaP and PEGylated AuNP@CaP were estimated with HeLa cells by MTT assay. Cell viability of PEGylated Dox-AuNP@CaP came down to 19% after 24 h incubation, while the PEGylated AuNP@CaP particle itself showed slight cytotoxicity with the same conditions. The prepared PEGylated Dox-AuNP@CaP worked as a pH- and ion concentration-sensitive drug-delivery system as well as theranostic nanoparticles for CT imaging and cancer treatment.

KW - Calcium phosphate

KW - Computed tomography (CT)

KW - Gold nanoparticle

KW - PH sensitive

KW - Theranostics

UR - http://www.scopus.com/inward/record.url?scp=84862185074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862185074&partnerID=8YFLogxK

U2 - 10.1007/s13233-012-0061-y

DO - 10.1007/s13233-012-0061-y

M3 - Article

AN - SCOPUS:84862185074

VL - 20

SP - 319

EP - 326

JO - Macromolecular Research

JF - Macromolecular Research

SN - 1598-5032

IS - 3

ER -