Development of a Saccharomyces cerevisiae strain for increasing the accumulation of triacylglycerol as a microbial oil feedstock for biodiesel production using glycerol as a substrate

Kyung Ok Yu, Ju Jung, Ahmad Bazli Ramzi, Se Hoon Choe, Seung Wook Kim, Chulhwan Park, Sung Ok Han

Research output: Contribution to journalLetterpeer-review

31 Citations (Scopus)

Abstract

Triacylglycerol (TAG) is a microbial oil feedstock for biodiesel production that uses an inexpensive substrate, such as glycerol. Here, we demonstrated the overproduction of TAG from glycerol in engineered Saccharomyces cerevisiae via the glycerol-3-phosphate (G3P) pathway by overexpressing the major TAG synthesis. The G3P accumulation was increased 2.4-fold with the increased glycerol utilization gained by the overexpression of glycerol kinase (GUT1). By overexpressing diacylglycerol acyltransferase (DGA1) and phospholipid diacylglycerol acyltransferase (LRO1), the engineered YPH499 (pGutDgaLro1) strain produced 23.0mg/L lipids, whereas the YPH499 (pESC-TRP) strain produced 6.2mg/L total lipids and showed a lipid content that was increased 1.4-fold compared with 3.6% for the wild-type strain after 96h of cultivation. After 96h of cultivation using glycerol, the overall content of TAG in the engineered strain, YPH499 (pGutDgaLro1), yielded 8.2% TAG, representing a 2.3-fold improvement, compared with 3.6% for the wild-type strain. The results should allow a reduction of costs and a more sustainable production of biodiesel.

Original languageEnglish
Pages (from-to)343-347
Number of pages5
JournalBiotechnology and Bioengineering
Volume110
Issue number1
DOIs
Publication statusPublished - 2013 Jan

Keywords

  • Glycerol
  • Glycerol-3-phosphate
  • Saccharomyces cerevisiae
  • TAG synthesis
  • Triacylgrycerol

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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