Development of a Saccharomyces cerevisiae strain for the production of 1,2-propanediol by gene manipulation

Eunyoung Jeon, Soojin Lee, Donghyun Kim, Hyunsik Yoon, Min-Kyu Oh, Chulhwan Park, Jinwon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The main goal of this research was to achieve a more efficient production of 1,2-propanediol (1,2-PD) using mutated Saccharomyces cerevisiae. 1,2-PD cannot be produced by wild type S. cerevisiae. To develop a S. cerevisiae mutant that could produce 1,2-PD, the mgs gene of E. coli-K12 MG1655 and the dhaD gene of Citrobacter freundii were inserted into yeast expression vectors such as pESC-URA and pESC-TRP and transformed into the wild type of S. cerevisiae. As a result, the batch fermentation of S. cerevisiae YPH500, harboring an mgs gene inserted pJES27 vector, resulted in a yield of 0.17 g/L. On the other hand, the methylglyoxal synthase of the recombinant S. cerevisiae YPH500, harboring a dhaD gene inserted pJES29 vector, was inactive and produced no detectable amount of 1,2-PD. Therefore, in order to achieve a maximum yield of 1,2-PD, we selected the pESC-TRP vector that is able to co-express the dhaD gene with the pJES27 vector. By inserting the dhaD gene into the pESC-TRP vector, the pJES30 vector was constructed. The maximal yield of 1,2-PD achieved in a 1% galactose batch fermentation by pJES27 and pJES30 harboring S. cerevisiae was 0.45 g/L.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalEnzyme and Microbial Technology
Volume45
Issue number1
DOIs
Publication statusPublished - 2009 Jul 8

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Propylene Glycol
Yeast
Saccharomyces cerevisiae
Genes
Fermentation
Citrobacter freundii
Escherichia coli K12
Galactose
Escherichia coli
Yeasts
Research

Keywords

  • 1,2-Propanediol
  • DhaD gene
  • Fermentation
  • Mgs gene
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Development of a Saccharomyces cerevisiae strain for the production of 1,2-propanediol by gene manipulation. / Jeon, Eunyoung; Lee, Soojin; Kim, Donghyun; Yoon, Hyunsik; Oh, Min-Kyu; Park, Chulhwan; Lee, Jinwon.

In: Enzyme and Microbial Technology, Vol. 45, No. 1, 08.07.2009, p. 42-47.

Research output: Contribution to journalArticle

Jeon, Eunyoung ; Lee, Soojin ; Kim, Donghyun ; Yoon, Hyunsik ; Oh, Min-Kyu ; Park, Chulhwan ; Lee, Jinwon. / Development of a Saccharomyces cerevisiae strain for the production of 1,2-propanediol by gene manipulation. In: Enzyme and Microbial Technology. 2009 ; Vol. 45, No. 1. pp. 42-47.
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