Development of a SCAR marker associated with salt tolerance in durum wheat (Triticum turgidum ssp. durum) from a semi-arid region

Sang Heon Kim, Jae Yoon Kim, Dae Yeon Kim, Jin Seok Yoon, Woo Joo Jung, Inés Yacoubi, Yong Weon Seo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Durum wheat (Triticum turgidum ssp. durum) is one of the main species of cultivated wheat. In arid and semi-arid areas, salinity stress reduces durum wheat productivity. This study used 26 durum wheat accessions from semi-arid regions in Tunisia to analyze plant tolerance to salt stress. Salt stress was experimentally applied by regularly submerging pots in NaCl solution. The salt tolerance trait index (STTI) and salt tolerance index (STI) of various growth parameters were used as criteria to select for salt tolerance. Analysis of genetic relationships was carried out to determine the genetic distance between durum wheat accessions. Based on simple sequence repeats analysis, a molecular marker for salt stress resistance in durum wheat was developed. Salt-treated plants had reduced morphological traits compared to control plants. Most STTIs in all genotypes were below 100 %. Based on STI, 8 accessions were found to be salt-resistant, 16 were salt-moderate, two were salt-susceptible. Analysis of the genetic relationships among 28 Tunisian durum wheat accessions revealed that landraces of the same nominal type are closely related. Of the 94 SSR primers investigated, three were selected and used to design sequence characterized amplified region (SCAR) primers. One SCAR primer pair, KUCMB_Xgwm403_2, produced a 207 bp band that was present in salt-resistant durum wheat lines but absent in salt-susceptible lines. The results suggest that KUCMB_Xgwm403_2 could be a potential genetic tag for salt-tolerant durum wheats.

Original languageEnglish
Pages (from-to)939-948
Number of pages10
JournalGenes and Genomics
Volume38
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

Salt-Tolerance
Arid regions
Triticum
Salts
Tunisia
Salinity
Microsatellite Repeats
Sequence Analysis
Genotype

Keywords

  • Durum wheat
  • Salt tolerance
  • SCAR
  • Semi-arid region
  • SSR
  • Triticum turgidum ssp. durum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Development of a SCAR marker associated with salt tolerance in durum wheat (Triticum turgidum ssp. durum) from a semi-arid region. / Kim, Sang Heon; Kim, Jae Yoon; Kim, Dae Yeon; Yoon, Jin Seok; Jung, Woo Joo; Yacoubi, Inés; Seo, Yong Weon.

In: Genes and Genomics, Vol. 38, No. 10, 01.10.2016, p. 939-948.

Research output: Contribution to journalArticle

Kim, Sang Heon ; Kim, Jae Yoon ; Kim, Dae Yeon ; Yoon, Jin Seok ; Jung, Woo Joo ; Yacoubi, Inés ; Seo, Yong Weon. / Development of a SCAR marker associated with salt tolerance in durum wheat (Triticum turgidum ssp. durum) from a semi-arid region. In: Genes and Genomics. 2016 ; Vol. 38, No. 10. pp. 939-948.
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