Development of a 68Ga-labelled PET tracer for carbonic anhydrase IX-overexpressed tumors using the artificial sweetener saccharin

Un chul Shin, Jeong Su Choi, Yeon Jae Beak, Min woo Lee, Hyung Soo Kim, DalWoong Choi, Dong Gil Kim, Suhng Wook Kim

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we developed a saccharin (SAC)-based radiopharmaceutical (68Ga-NOTA-SAC) and evaluated the possibility of its application as a PET tracer in the diagnosis of carbonic anhydrase IX (CA IX)-overexpressed tumors. We did a water-soluble tetrazolium assay and flow cytometry analysis to identify the cell viability decrease by SAC. The radiochemical purity and stability of 68Ga- NOTA-SAC in human and mouse serum was greater than 98%. The small animal PET image-based radioactivity distribution of all organs decreased over time.68Ga-NOTA-SAC presented the highest tumor-to-muscle ratio at 90 min post injection (p.i). The growth rates of tumor-to-muscle ratios of 68Ga-NOTA-SAC were 88% at 60 min and 220% at 90 min, compared to 30 min p.i. The potential of 68Ga-NOTA-SAC as a PET tracer is expected to contribute to the diagnostic research on CA IX-overexpressed tumors with the advantages of a relatively simple synthesis method.

Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalJournal of Labelled Compounds and Radiopharmaceuticals
Volume64
Issue number3
DOIs
Publication statusPublished - 2021 Mar

Keywords

  • Carbonic anhydrase IX
  • Ga-NOTA-SAC
  • PET tracer
  • Saccharin
  • U87MG

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Radiology Nuclear Medicine and imaging
  • Drug Discovery
  • Spectroscopy
  • Organic Chemistry

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