Development of a web-based program for the identification of human leukocyte antigen antibody specificities

Choong Hwan Cha, Heung Bum Oh, Myeong Hee Kim, Jeong Min Chae, Soon Young Jung

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3 Citations (Scopus)

Abstract

BACKGROUND: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but it is very difficult to perform manually. METHODS: To develop a web-based program, PHP (5.1.2), Apache (2.0.55), and MySQL (5.0.22) were used. Tail analysis algorithm was applied to identify specificities, which analyzed statistically 2 x 2 tables representing reactivities to broad antigens, splits and cross reactive groups (CREG). Exploiting two CREG classifications of Rodey (R) and Takemoto (T), antibody specificities were identified by 3 methods (ABC, R-ABC, T-ABC) simultaneously. Performance of the system was evaluated using 159 samples that showed > or =6 PRA% by a lymphocytotoxicity assay. RESULTS: A web-based system that can identify HLA antibody specificities was implemented on www.koreanhla.com. Among 159 samples tested, antibody specificities were identified in 151 (95.0 %), but not in 8 samples with PRA >97%. Among the 151 samples, 110 showed broad or split specificities and 41 CREG specificities. CONCLUSIONS: We developed a web-based computer program for the identification of HLA antibody specificities. Accessible to everyone on the internet, this program should be of help in sharing PRA results among laboratories.

Original languageEnglish
Pages (from-to)458-463
Number of pages6
JournalThe Korean journal of laboratory medicine
Volume27
Issue number6
DOIs
Publication statusPublished - 2007 Dec 1
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry

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