Development of a web-based program for the identification of human leukocyte antigen antibody specificities

Choong Hwan Cha, Heung Bum Oh, Myeong Hee Kim, Jeong Min Chae, Soon Young Jung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

BACKGROUND: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but it is very difficult to perform manually. METHODS: To develop a web-based program, PHP (5.1.2), Apache (2.0.55), and MySQL (5.0.22) were used. Tail analysis algorithm was applied to identify specificities, which analyzed statistically 2 x 2 tables representing reactivities to broad antigens, splits and cross reactive groups (CREG). Exploiting two CREG classifications of Rodey (R) and Takemoto (T), antibody specificities were identified by 3 methods (ABC, R-ABC, T-ABC) simultaneously. Performance of the system was evaluated using 159 samples that showed > or =6 PRA% by a lymphocytotoxicity assay. RESULTS: A web-based system that can identify HLA antibody specificities was implemented on www.koreanhla.com. Among 159 samples tested, antibody specificities were identified in 151 (95.0 %), but not in 8 samples with PRA >97%. Among the 151 samples, 110 showed broad or split specificities and 41 CREG specificities. CONCLUSIONS: We developed a web-based computer program for the identification of HLA antibody specificities. Accessible to everyone on the internet, this program should be of help in sharing PRA results among laboratories.

Original languageEnglish
Pages (from-to)458-463
Number of pages6
JournalThe Korean journal of laboratory medicine
Volume27
Issue number6
DOIs
Publication statusPublished - 2007 Dec 1
Externally publishedYes

Fingerprint

Antibody Specificity
HLA Antigens
Antibodies
Internet
Software
Transplantation
Antigens
Computer program listings
Assays

ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry

Cite this

Development of a web-based program for the identification of human leukocyte antigen antibody specificities. / Cha, Choong Hwan; Oh, Heung Bum; Kim, Myeong Hee; Chae, Jeong Min; Jung, Soon Young.

In: The Korean journal of laboratory medicine, Vol. 27, No. 6, 01.12.2007, p. 458-463.

Research output: Contribution to journalArticle

Cha, Choong Hwan ; Oh, Heung Bum ; Kim, Myeong Hee ; Chae, Jeong Min ; Jung, Soon Young. / Development of a web-based program for the identification of human leukocyte antigen antibody specificities. In: The Korean journal of laboratory medicine. 2007 ; Vol. 27, No. 6. pp. 458-463.
@article{0c0610f1269146e0980fe49ae35fee25,
title = "Development of a web-based program for the identification of human leukocyte antigen antibody specificities",
abstract = "BACKGROUND: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but it is very difficult to perform manually. METHODS: To develop a web-based program, PHP (5.1.2), Apache (2.0.55), and MySQL (5.0.22) were used. Tail analysis algorithm was applied to identify specificities, which analyzed statistically 2 x 2 tables representing reactivities to broad antigens, splits and cross reactive groups (CREG). Exploiting two CREG classifications of Rodey (R) and Takemoto (T), antibody specificities were identified by 3 methods (ABC, R-ABC, T-ABC) simultaneously. Performance of the system was evaluated using 159 samples that showed > or =6 PRA{\%} by a lymphocytotoxicity assay. RESULTS: A web-based system that can identify HLA antibody specificities was implemented on www.koreanhla.com. Among 159 samples tested, antibody specificities were identified in 151 (95.0 {\%}), but not in 8 samples with PRA >97{\%}. Among the 151 samples, 110 showed broad or split specificities and 41 CREG specificities. CONCLUSIONS: We developed a web-based computer program for the identification of HLA antibody specificities. Accessible to everyone on the internet, this program should be of help in sharing PRA results among laboratories.",
author = "Cha, {Choong Hwan} and Oh, {Heung Bum} and Kim, {Myeong Hee} and Chae, {Jeong Min} and Jung, {Soon Young}",
year = "2007",
month = "12",
day = "1",
doi = "10.3343/kjlm.2007.27.6.458",
language = "English",
volume = "27",
pages = "458--463",
journal = "Annals of Laboratory Medicine",
issn = "2234-3806",
publisher = "Seoul National University",
number = "6",

}

TY - JOUR

T1 - Development of a web-based program for the identification of human leukocyte antigen antibody specificities

AU - Cha, Choong Hwan

AU - Oh, Heung Bum

AU - Kim, Myeong Hee

AU - Chae, Jeong Min

AU - Jung, Soon Young

PY - 2007/12/1

Y1 - 2007/12/1

N2 - BACKGROUND: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but it is very difficult to perform manually. METHODS: To develop a web-based program, PHP (5.1.2), Apache (2.0.55), and MySQL (5.0.22) were used. Tail analysis algorithm was applied to identify specificities, which analyzed statistically 2 x 2 tables representing reactivities to broad antigens, splits and cross reactive groups (CREG). Exploiting two CREG classifications of Rodey (R) and Takemoto (T), antibody specificities were identified by 3 methods (ABC, R-ABC, T-ABC) simultaneously. Performance of the system was evaluated using 159 samples that showed > or =6 PRA% by a lymphocytotoxicity assay. RESULTS: A web-based system that can identify HLA antibody specificities was implemented on www.koreanhla.com. Among 159 samples tested, antibody specificities were identified in 151 (95.0 %), but not in 8 samples with PRA >97%. Among the 151 samples, 110 showed broad or split specificities and 41 CREG specificities. CONCLUSIONS: We developed a web-based computer program for the identification of HLA antibody specificities. Accessible to everyone on the internet, this program should be of help in sharing PRA results among laboratories.

AB - BACKGROUND: Panel reactive antibody (PRA) test is used to determine whether a patient awaiting transplantation is previously sensitized. Tail analysis algorithm is widely used to identify antibody specificities, but it is very difficult to perform manually. METHODS: To develop a web-based program, PHP (5.1.2), Apache (2.0.55), and MySQL (5.0.22) were used. Tail analysis algorithm was applied to identify specificities, which analyzed statistically 2 x 2 tables representing reactivities to broad antigens, splits and cross reactive groups (CREG). Exploiting two CREG classifications of Rodey (R) and Takemoto (T), antibody specificities were identified by 3 methods (ABC, R-ABC, T-ABC) simultaneously. Performance of the system was evaluated using 159 samples that showed > or =6 PRA% by a lymphocytotoxicity assay. RESULTS: A web-based system that can identify HLA antibody specificities was implemented on www.koreanhla.com. Among 159 samples tested, antibody specificities were identified in 151 (95.0 %), but not in 8 samples with PRA >97%. Among the 151 samples, 110 showed broad or split specificities and 41 CREG specificities. CONCLUSIONS: We developed a web-based computer program for the identification of HLA antibody specificities. Accessible to everyone on the internet, this program should be of help in sharing PRA results among laboratories.

UR - http://www.scopus.com/inward/record.url?scp=58149088085&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149088085&partnerID=8YFLogxK

U2 - 10.3343/kjlm.2007.27.6.458

DO - 10.3343/kjlm.2007.27.6.458

M3 - Article

C2 - 18160837

AN - SCOPUS:58149088085

VL - 27

SP - 458

EP - 463

JO - Annals of Laboratory Medicine

JF - Annals of Laboratory Medicine

SN - 2234-3806

IS - 6

ER -