Development of AFLP and STS markers for identifying wheat-rye translocations possessing 2RL

Yong Weon Seo, Cheol Seong Jang, Jerry W. Johnson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Wheat-rye translocation line (2BS/2RL) has been developed for resistance to biotype L of Hessian fly and agronomically useful traits. AFLP analysis using 64 primer pairs was conducted in order to identify 2RL-specific polymorphisms between 'Coker 797' (non-2RL), near-isogenic line (NIL) carrying 2RL, and 'Hamlet'. Nine primer combinations identified twelve reproducible polymorphic fragments in the NIL carrying 2RL. These twelve fragments were cloned and sequenced with an aim towards converting AFLP markers into sequence tagged sites (STS). A comparison of the 12 sequences with non-redundant accessions in the NCBI database using the BLAST search option indicated that one fragment of approximately 200 bp in length (amplified using primer combination E+AAC / M+CTA) was highly homologous with the rye-specific repetitive sequence R173-1 and Wis-2-1A, a retrotransposon-like element in wheat. Two STS primers (SJ07 and SJ09) out of twelve STS primer sets enabled the detection of polymorphisms between Coker 797 and NIL carrying 2RL. In order to verify whether the polymorphism detected by primers SJ07 and SJ09 was in fact the result of the presence of 2RL, additional plant material was examined. Amplified products of about 260 bp fragment with the SJ07 primer set were generated in rye cvs. 'Chilbohomil' and 'Jochunhomil', triticale experimental line Suwon 15, and wheat experimental line K-14 (1AL/1RS & 2BS/2RL), as well as NIL carrying 2RL and Hamlet, but not in Coker 797 (non-2RL), 'Keumgangmil' (non-translocation wheat), KS92WGRC17 (PI592729/;/6BS/6BL6RL), KS92WGRC19 (P1592731/;/4BS/4BL6RL), 'TAM200' (1AL/1RS), and 'Siouxland' (1BL/1RS). Our data suggest that primer set SJ07 amplifies a '2RL-specific' fragment of diagnostic value.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalEuphytica
Volume121
Issue number3
DOIs
Publication statusPublished - 2001 Dec 24

Fingerprint

Sequence Tagged Sites
isogenic lines
rye
Triticum
amplified fragment length polymorphism
wheat
genetic polymorphism
Amplified Fragment Length Polymorphism Analysis
Mayetiola destructor
translocation lines
Retroelements
repetitive sequences
Nucleic Acid Repetitive Sequences
triticale
retrotransposons
disease diagnosis
biotypes
Diptera
Databases
Secale

Keywords

  • 2BS/2RL
  • AFLP
  • H21
  • Molecular markers
  • STS
  • Wheat-rye translocation

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science

Cite this

Development of AFLP and STS markers for identifying wheat-rye translocations possessing 2RL. / Seo, Yong Weon; Jang, Cheol Seong; Johnson, Jerry W.

In: Euphytica, Vol. 121, No. 3, 24.12.2001, p. 279-287.

Research output: Contribution to journalArticle

Seo, Yong Weon ; Jang, Cheol Seong ; Johnson, Jerry W. / Development of AFLP and STS markers for identifying wheat-rye translocations possessing 2RL. In: Euphytica. 2001 ; Vol. 121, No. 3. pp. 279-287.
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AB - Wheat-rye translocation line (2BS/2RL) has been developed for resistance to biotype L of Hessian fly and agronomically useful traits. AFLP analysis using 64 primer pairs was conducted in order to identify 2RL-specific polymorphisms between 'Coker 797' (non-2RL), near-isogenic line (NIL) carrying 2RL, and 'Hamlet'. Nine primer combinations identified twelve reproducible polymorphic fragments in the NIL carrying 2RL. These twelve fragments were cloned and sequenced with an aim towards converting AFLP markers into sequence tagged sites (STS). A comparison of the 12 sequences with non-redundant accessions in the NCBI database using the BLAST search option indicated that one fragment of approximately 200 bp in length (amplified using primer combination E+AAC / M+CTA) was highly homologous with the rye-specific repetitive sequence R173-1 and Wis-2-1A, a retrotransposon-like element in wheat. Two STS primers (SJ07 and SJ09) out of twelve STS primer sets enabled the detection of polymorphisms between Coker 797 and NIL carrying 2RL. In order to verify whether the polymorphism detected by primers SJ07 and SJ09 was in fact the result of the presence of 2RL, additional plant material was examined. Amplified products of about 260 bp fragment with the SJ07 primer set were generated in rye cvs. 'Chilbohomil' and 'Jochunhomil', triticale experimental line Suwon 15, and wheat experimental line K-14 (1AL/1RS & 2BS/2RL), as well as NIL carrying 2RL and Hamlet, but not in Coker 797 (non-2RL), 'Keumgangmil' (non-translocation wheat), KS92WGRC17 (PI592729/;/6BS/6BL6RL), KS92WGRC19 (P1592731/;/4BS/4BL6RL), 'TAM200' (1AL/1RS), and 'Siouxland' (1BL/1RS). Our data suggest that primer set SJ07 amplifies a '2RL-specific' fragment of diagnostic value.

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