Development of an effective sample preparation method for the proteome analysis of body fluids using 2-D gel electrophoresis

Won A. Joo, Do Youn Lee, Chan Wha Kim

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained.

Original languageEnglish
Pages (from-to)1574-1577
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number7
Publication statusPublished - 2003 Jul 1

Keywords

  • Body fluids
  • Centrifugal filter device, detergent
  • IEF
  • Sample preparation of 2-DE

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

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