Development of an effective sample preparation method for the proteome analysis of body fluids using 2-d gel electrophoresis

Won A. Joo, Do Youn Lee, Chan Wha Kim

Research output: Contribution to journalArticlepeer-review

51 Citations (Scopus)

Abstract

A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained.

Original languageEnglish
Pages (from-to)1574-1577
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number7
DOIs
Publication statusPublished - 2003 Jan 1

Keywords

  • Body fluids
  • Centrifugal filter device, detergent
  • IEF
  • Sample preparation of 2-DE

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Development of an effective sample preparation method for the proteome analysis of body fluids using 2-d gel electrophoresis'. Together they form a unique fingerprint.

Cite this