Development of an effective sample preparation method for the proteome analysis of body fluids using 2-D gel electrophoresis

Won A. Joo, Do Youn Lee, Chan Wha Kim

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A sample preparation is still the most critical step in two-dimensional electrophoresis (2-DE) and should be optimized for each type of sample. In this study, a protein extraction method from body fluids was developed using a combined centrifugal filter device and a sample treating buffer. When plasma, amniotic fluid, urine, and tear were tested with this method, the recovery of protein reached almost 90% and high-quality separation of 2-DE gel was obtained.

Original languageEnglish
Pages (from-to)1574-1577
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume67
Issue number7
Publication statusPublished - 2003 Jul 1

Fingerprint

body fluids
Body fluids
Electrophoresis, Gel, Two-Dimensional
Body Fluids
Proteome
proteome
Electrophoresis
gel electrophoresis
Gels
Proteins
Amniotic Fluid
Tears
amniotic fluid
Buffers
Urine
Plasmas
Recovery
sampling
Equipment and Supplies
electrophoresis

Keywords

  • Body fluids
  • Centrifugal filter device, detergent
  • IEF
  • Sample preparation of 2-DE

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology

Cite this

Development of an effective sample preparation method for the proteome analysis of body fluids using 2-D gel electrophoresis. / Joo, Won A.; Lee, Do Youn; Kim, Chan Wha.

In: Bioscience, Biotechnology and Biochemistry, Vol. 67, No. 7, 01.07.2003, p. 1574-1577.

Research output: Contribution to journalArticle

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