Development of anti-biofouling interface on hydroxyapatite surface by coating zwitterionic MPC polymer containing calcium-binding moieties to prevent oral bacterial adhesion

Sunah Kang, Myoungjin Lee, Minji Kang, Minwoo Noh, Joohee Jeon, Yan Lee, Ji-Hun Seo

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The purpose of the present study is to synthesize a 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer capable of being immobilized on the tooth surface to prevent oral bacterial adhesion. The strategy is to develop an MPC-based polymer with Ca2+-binding moieties, i.e., phosphomonoester groups, for stronger binding with hydroxyapatite (HA) of the tooth surface. To this end, a 2-methacryloyloxyethyl phosphate (MOEP) monomer was synthesized and copolymerized with MPC by free radical polymerization. The coating efficiency of the synthesized polymer, MPC-ran-MOEP (abbreviated as PMP) with varied composition, onto a HA surface was estimated by means of contact angle measurement and X-ray photoelectron spectroscopy. The anti-biofouling nature of PMP-coated HA surfaces was estimated by analyzing protein adsorption, cell adhesion, and Streptococcus mutans adhesion. As a result, HA surface coated with a copolymer containing around 50% MPC (PMP50) showed the best performance in preventing protein adsorption and the downstream cell and bacterial adhesion. Statement of Significance Preparation of anti-biofouling surface on the tooth enamel is the key technique to prevent dental and periodontal diseases, which are closely related with the biofilm formation that induced by the adsorption of salivary proteins and the adhesion of oral bacteria on the tooth surface. In this research, a PMP copolymer with an optimized ratio of zwitterionic and Ca2+-binding moieties could form a highly effective and robust anti-biofouling surface on HA surfaces by a simple coating method. The PMP-coated surface with high stability can provide a new strategy for an anti-adsorptive and anti-bacterial platform in dentistry and related fields.

Original languageEnglish
Pages (from-to)70-77
Number of pages8
JournalActa Biomaterialia
Volume40
DOIs
Publication statusPublished - 2016 Aug 1

Fingerprint

Biofouling
Bacterial Adhesion
Durapatite
Hydroxyapatite
Calcium
Polymers
Adhesion
Tooth
Coatings
Adsorption
Cell Adhesion
Phosphates
Salivary Proteins and Peptides
Stomatognathic Diseases
Photoelectron Spectroscopy
Streptococcus mutans
Periodontal Diseases
Dental Enamel
Biofilms
Dentistry

Keywords

  • 2-Methacryloyloxyethyl phosphorylcholine
  • Anti-bacterial adhesion
  • Anti-fouling
  • Calcium binding
  • Dental materials
  • Streptococcus mutans

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

Cite this

Development of anti-biofouling interface on hydroxyapatite surface by coating zwitterionic MPC polymer containing calcium-binding moieties to prevent oral bacterial adhesion. / Kang, Sunah; Lee, Myoungjin; Kang, Minji; Noh, Minwoo; Jeon, Joohee; Lee, Yan; Seo, Ji-Hun.

In: Acta Biomaterialia, Vol. 40, 01.08.2016, p. 70-77.

Research output: Contribution to journalArticle

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abstract = "The purpose of the present study is to synthesize a 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer capable of being immobilized on the tooth surface to prevent oral bacterial adhesion. The strategy is to develop an MPC-based polymer with Ca2+-binding moieties, i.e., phosphomonoester groups, for stronger binding with hydroxyapatite (HA) of the tooth surface. To this end, a 2-methacryloyloxyethyl phosphate (MOEP) monomer was synthesized and copolymerized with MPC by free radical polymerization. The coating efficiency of the synthesized polymer, MPC-ran-MOEP (abbreviated as PMP) with varied composition, onto a HA surface was estimated by means of contact angle measurement and X-ray photoelectron spectroscopy. The anti-biofouling nature of PMP-coated HA surfaces was estimated by analyzing protein adsorption, cell adhesion, and Streptococcus mutans adhesion. As a result, HA surface coated with a copolymer containing around 50{\%} MPC (PMP50) showed the best performance in preventing protein adsorption and the downstream cell and bacterial adhesion. Statement of Significance Preparation of anti-biofouling surface on the tooth enamel is the key technique to prevent dental and periodontal diseases, which are closely related with the biofilm formation that induced by the adsorption of salivary proteins and the adhesion of oral bacteria on the tooth surface. In this research, a PMP copolymer with an optimized ratio of zwitterionic and Ca2+-binding moieties could form a highly effective and robust anti-biofouling surface on HA surfaces by a simple coating method. The PMP-coated surface with high stability can provide a new strategy for an anti-adsorptive and anti-bacterial platform in dentistry and related fields.",
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