Development of batch and continuous processes on biodiesel production in a packed-bed reactor by a mixture of immobilized Candida rugosa and Rhizopus oryzae lipases

Jong Ho Lee, Sung Bong Kim, Chulhwan Park, Bumseok Tae, Sung Ok Han, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

In this study, transesterification and esterification were investigated in batch and continuous process using immobilized Candida rugosa and Rhizopus oryzae lipases. In the case of batch process, stepwise reaction method was investigated to prevent the lipase deactivation. Reaction conditions were as follows: temperature, 45 °C; agitation speed, 250 rpm; enzyme concentration, 20%; and water contents 10%. And then, conversion yield was 98.33% at 4 h. In the case of continuous process, circulation and long-term continuous system were investigated for development of efficient mass transfer system. Optimal reaction conditions were as follows: temperature, 45 °C; flow rate, 0.8 mL/min; and water contents, 10%. And then, conversion yield of biodiesel was 97.98% at 3 h. Especially, the maximum conversion yield using a mixture of immobilized lipases exceeded over 90% for 108 h in longterm continuous system under optimal reaction conditions (45 °C; flow rate, 0.8 mL/min; and water contents, 10%). These results should help in determining the bestmethod for the biodiesel production and improving the design and operation of large scale by enzymatic systems.

Original languageEnglish
Pages (from-to)365-371
Number of pages7
JournalApplied Biochemistry and Biotechnology
Volume161
Issue number1-8
DOIs
Publication statusPublished - 2010 May

Keywords

  • Circulation process
  • Esterification
  • Long-term continuous process
  • Packed-bed reactor
  • Transesterification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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