Development of bio-nanowire networks using phage-enabled assembly for biological sensor application

Yu R. Kang, Eun Jin Park, Ju Hwan Kim, Nam K. Min, Soo-Won Kim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This paper proposes a new approach to detect an electrochemical reaction using a working area consisting of bio-nanowires from genetically modified filamentous phages and nanoparticles. Use of the nanomaterials on the working electrode is a vital consideration in biological sensor development, because the biosensor sensitivity heavily depends on the material used. Here we use that fd-tet p8MMM filamentous phages displaying the MMM peptide on the major coat protein pVIII (designated p8MMM phages) were immobilized on the active area of an electrochemical sensor through chemical binding. The bio-nanowires composed of p8MMM phages and silver nanoparticles facilitated sensitive, rapid detection of particular molecules. We performed the experiment for observing electrochemical glucose detection to estimate the possibility of using one or other characterized-biological sensor. The current response of the bio-nanowire sensor reached sufficiently high signals at various glucose concentrations (10-7 to 10-4 M). The cyclic voltammetry peak current Ip and peak potential Ep were 689 μA/cm2 and 280 mV, respectively. The filamentous nanophage-based electrode displayed a high sensitivity and good stability under various pH and temperature in enzyme determination. As a result, it may have wide application in analytical systems, label-free detection and biological sensors.

Original languageEnglish
Pages (from-to)1425-1430
Number of pages6
JournalTalanta
Volume81
Issue number4-5
DOIs
Publication statusPublished - 2010 Jun 15

Fingerprint

Nanowires
Bacteriophages
Sensors
Nanoparticles
Electrodes
Glucose
Electrochemical sensors
Nanostructures
Capsid Proteins
Biosensing Techniques
Silver
Nanostructured materials
Biosensors
Cyclic voltammetry
Labels
Peptides
Molecules
Temperature
Enzymes
Experiments

Keywords

  • Biological sensor application
  • Electrochemical detection
  • Electrode modification
  • Filamentous phage
  • Label-free detection
  • Nucleophile-mediated silver binding

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Development of bio-nanowire networks using phage-enabled assembly for biological sensor application. / Kang, Yu R.; Park, Eun Jin; Kim, Ju Hwan; Min, Nam K.; Kim, Soo-Won.

In: Talanta, Vol. 81, No. 4-5, 15.06.2010, p. 1425-1430.

Research output: Contribution to journalArticle

Kang, Yu R. ; Park, Eun Jin ; Kim, Ju Hwan ; Min, Nam K. ; Kim, Soo-Won. / Development of bio-nanowire networks using phage-enabled assembly for biological sensor application. In: Talanta. 2010 ; Vol. 81, No. 4-5. pp. 1425-1430.
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