Development of complete thoracic spinal cord transection model in rats for delayed transplantation of stem cells

Sang Hyuk Min, Sang Hoon Lee, Hosup Shim, Jeong Soo Park, Young Ii Lee, Hae Won Kim, Jung Keun Hyun

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Study Design.: In vivo study of a rat spinal cord injury model. Objectives.: To develop complete transection model of thoracic spinal cord using a polymer sheet and a microtube relevant for delayed transplantation of stem cells. Summary of Background Data.: Stem cell transplantation for the regeneration of spinal cord injuries has used animal models. However, current models suffer from inflammation and leakage, which lessens their usefulness in studying delayed stem cell transplantation. Methods.: Thoracic spinal cord at T9 level of adult Sprague-Dawley rats was exposed and a 50:50 sheet of poly(D,L-lactic-coglycolic acid) was inserted, exposed spinal cord was completely transected, and collagen was filled between the gap between the proximal and distal stumps of transected spinal cord. A microtube was placed and fixed between the polymer surfaces facing each other. Behavior testing, magnetic resonance imaging, and myelography were performed to characterize the new complete transection with a gap formation and polymer insertion (GAP) model and to compare the GAP model with the control models. Human mesenchymal stem cells (hMSCs) were transplanted into 3 models and immunohistochemistry and western blot were performed. Results.: The inserted poly(D,L-lactic-coglycolic acid) sheet was completely disappeared 10 weeks after operation, but the inserted microtube remained firmly fixed in its original position. Myelography of the GAP model showed no leakage of contrast medium around the injured spinal cord, whereas magnetic resonance imaging of the severe contusion and simple transection models showed some leakage of contrast medium. Immunohistochemistry and western blot after hMSCs transplantation indicated that transplanted hMSCs survived and migrated well in the GAP model, and the deposition of inflammatory cells in GAP model was less than a simple transection model or severe contusion model. Conclusion.: The developed GAP model is more relevant for delayed transplantation of stem cells for the study of regeneration of spinal cord injury of rats.

Original languageEnglish
JournalSpine
Volume36
Issue number3
DOIs
Publication statusPublished - 2011 Feb 1

Fingerprint

Stem Cell Transplantation
Spinal Cord Injuries
Spinal Cord
Thorax
Myelography
Contusions
Mesenchymal Stromal Cells
Contrast Media
Regeneration
Lactic Acid
Polymers
Western Blotting
Immunohistochemistry
Magnetic Resonance Imaging
Mesenchymal Stem Cell Transplantation
Sprague Dawley Rats
Collagen
Animal Models
Inflammation

Keywords

  • infl ammation
  • microtube
  • polymer
  • Spinal cord injury
  • transection model
  • transplantation

ASJC Scopus subject areas

  • Clinical Neurology
  • Orthopedics and Sports Medicine

Cite this

Development of complete thoracic spinal cord transection model in rats for delayed transplantation of stem cells. / Min, Sang Hyuk; Lee, Sang Hoon; Shim, Hosup; Park, Jeong Soo; Lee, Young Ii; Kim, Hae Won; Hyun, Jung Keun.

In: Spine, Vol. 36, No. 3, 01.02.2011.

Research output: Contribution to journalArticle

Min, Sang Hyuk ; Lee, Sang Hoon ; Shim, Hosup ; Park, Jeong Soo ; Lee, Young Ii ; Kim, Hae Won ; Hyun, Jung Keun. / Development of complete thoracic spinal cord transection model in rats for delayed transplantation of stem cells. In: Spine. 2011 ; Vol. 36, No. 3.
@article{c1dd79e68eff40089ed94fb99473ed01,
title = "Development of complete thoracic spinal cord transection model in rats for delayed transplantation of stem cells",
abstract = "Study Design.: In vivo study of a rat spinal cord injury model. Objectives.: To develop complete transection model of thoracic spinal cord using a polymer sheet and a microtube relevant for delayed transplantation of stem cells. Summary of Background Data.: Stem cell transplantation for the regeneration of spinal cord injuries has used animal models. However, current models suffer from inflammation and leakage, which lessens their usefulness in studying delayed stem cell transplantation. Methods.: Thoracic spinal cord at T9 level of adult Sprague-Dawley rats was exposed and a 50:50 sheet of poly(D,L-lactic-coglycolic acid) was inserted, exposed spinal cord was completely transected, and collagen was filled between the gap between the proximal and distal stumps of transected spinal cord. A microtube was placed and fixed between the polymer surfaces facing each other. Behavior testing, magnetic resonance imaging, and myelography were performed to characterize the new complete transection with a gap formation and polymer insertion (GAP) model and to compare the GAP model with the control models. Human mesenchymal stem cells (hMSCs) were transplanted into 3 models and immunohistochemistry and western blot were performed. Results.: The inserted poly(D,L-lactic-coglycolic acid) sheet was completely disappeared 10 weeks after operation, but the inserted microtube remained firmly fixed in its original position. Myelography of the GAP model showed no leakage of contrast medium around the injured spinal cord, whereas magnetic resonance imaging of the severe contusion and simple transection models showed some leakage of contrast medium. Immunohistochemistry and western blot after hMSCs transplantation indicated that transplanted hMSCs survived and migrated well in the GAP model, and the deposition of inflammatory cells in GAP model was less than a simple transection model or severe contusion model. Conclusion.: The developed GAP model is more relevant for delayed transplantation of stem cells for the study of regeneration of spinal cord injury of rats.",
keywords = "infl ammation, microtube, polymer, Spinal cord injury, transection model, transplantation",
author = "Min, {Sang Hyuk} and Lee, {Sang Hoon} and Hosup Shim and Park, {Jeong Soo} and Lee, {Young Ii} and Kim, {Hae Won} and Hyun, {Jung Keun}",
year = "2011",
month = "2",
day = "1",
doi = "10.1097/BRS.0b013e3181d8b92a",
language = "English",
volume = "36",
journal = "Spine",
issn = "0362-2436",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Development of complete thoracic spinal cord transection model in rats for delayed transplantation of stem cells

AU - Min, Sang Hyuk

AU - Lee, Sang Hoon

AU - Shim, Hosup

AU - Park, Jeong Soo

AU - Lee, Young Ii

AU - Kim, Hae Won

AU - Hyun, Jung Keun

PY - 2011/2/1

Y1 - 2011/2/1

N2 - Study Design.: In vivo study of a rat spinal cord injury model. Objectives.: To develop complete transection model of thoracic spinal cord using a polymer sheet and a microtube relevant for delayed transplantation of stem cells. Summary of Background Data.: Stem cell transplantation for the regeneration of spinal cord injuries has used animal models. However, current models suffer from inflammation and leakage, which lessens their usefulness in studying delayed stem cell transplantation. Methods.: Thoracic spinal cord at T9 level of adult Sprague-Dawley rats was exposed and a 50:50 sheet of poly(D,L-lactic-coglycolic acid) was inserted, exposed spinal cord was completely transected, and collagen was filled between the gap between the proximal and distal stumps of transected spinal cord. A microtube was placed and fixed between the polymer surfaces facing each other. Behavior testing, magnetic resonance imaging, and myelography were performed to characterize the new complete transection with a gap formation and polymer insertion (GAP) model and to compare the GAP model with the control models. Human mesenchymal stem cells (hMSCs) were transplanted into 3 models and immunohistochemistry and western blot were performed. Results.: The inserted poly(D,L-lactic-coglycolic acid) sheet was completely disappeared 10 weeks after operation, but the inserted microtube remained firmly fixed in its original position. Myelography of the GAP model showed no leakage of contrast medium around the injured spinal cord, whereas magnetic resonance imaging of the severe contusion and simple transection models showed some leakage of contrast medium. Immunohistochemistry and western blot after hMSCs transplantation indicated that transplanted hMSCs survived and migrated well in the GAP model, and the deposition of inflammatory cells in GAP model was less than a simple transection model or severe contusion model. Conclusion.: The developed GAP model is more relevant for delayed transplantation of stem cells for the study of regeneration of spinal cord injury of rats.

AB - Study Design.: In vivo study of a rat spinal cord injury model. Objectives.: To develop complete transection model of thoracic spinal cord using a polymer sheet and a microtube relevant for delayed transplantation of stem cells. Summary of Background Data.: Stem cell transplantation for the regeneration of spinal cord injuries has used animal models. However, current models suffer from inflammation and leakage, which lessens their usefulness in studying delayed stem cell transplantation. Methods.: Thoracic spinal cord at T9 level of adult Sprague-Dawley rats was exposed and a 50:50 sheet of poly(D,L-lactic-coglycolic acid) was inserted, exposed spinal cord was completely transected, and collagen was filled between the gap between the proximal and distal stumps of transected spinal cord. A microtube was placed and fixed between the polymer surfaces facing each other. Behavior testing, magnetic resonance imaging, and myelography were performed to characterize the new complete transection with a gap formation and polymer insertion (GAP) model and to compare the GAP model with the control models. Human mesenchymal stem cells (hMSCs) were transplanted into 3 models and immunohistochemistry and western blot were performed. Results.: The inserted poly(D,L-lactic-coglycolic acid) sheet was completely disappeared 10 weeks after operation, but the inserted microtube remained firmly fixed in its original position. Myelography of the GAP model showed no leakage of contrast medium around the injured spinal cord, whereas magnetic resonance imaging of the severe contusion and simple transection models showed some leakage of contrast medium. Immunohistochemistry and western blot after hMSCs transplantation indicated that transplanted hMSCs survived and migrated well in the GAP model, and the deposition of inflammatory cells in GAP model was less than a simple transection model or severe contusion model. Conclusion.: The developed GAP model is more relevant for delayed transplantation of stem cells for the study of regeneration of spinal cord injury of rats.

KW - infl ammation

KW - microtube

KW - polymer

KW - Spinal cord injury

KW - transection model

KW - transplantation

UR - http://www.scopus.com/inward/record.url?scp=79551536268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551536268&partnerID=8YFLogxK

U2 - 10.1097/BRS.0b013e3181d8b92a

DO - 10.1097/BRS.0b013e3181d8b92a

M3 - Article

C2 - 21124262

AN - SCOPUS:79551536268

VL - 36

JO - Spine

JF - Spine

SN - 0362-2436

IS - 3

ER -