Development of electrochemical biosensor for detection of pathogenic microorganism in Asian dust events

Min Sang Yoo, Minguk Shin, Younghun Kim, Min Jang, Yoon-E Choi, Si Jae Park, Jonghoon Choi, Jinyoung Lee, Chulhwan Park

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We developed a single-walled carbon nanotubes (SWCNTs)-based electrochemical biosensor for the detection of Bacillus subtilis, one of the microorganisms observed in Asian dust events, which causes respiratory diseases such as asthma and pneumonia. SWCNTs plays the role of a transducer in biological antigen/antibody reaction for the electrical signal while 1-pyrenebutanoic acid succinimidyl ester (1-PBSE) and ant-B. subtilis were performed as a chemical linker and an acceptor, respectively, for the adhesion of target microorganism in the developed biosensor. The detection range (10 2 –10 10  CFU/mL) and the detection limit (10 2  CFU/mL) of the developed biosensor were identified while the response time was 10 min. The amount of target B. subtilis was the highest in the specificity test of the developed biosensor, compared with the other tested microorganisms (Staphylococcus aureus, Flavobacterium psychrolimnae, and Aquabacterium commune). In addition, target B. subtilis detected by the developed biosensor was observed by scanning electron microscope (SEM) analysis.

Original languageEnglish
Pages (from-to)269-274
Number of pages6
JournalChemosphere
Volume175
DOIs
Publication statusPublished - 2017 Jan 1

Keywords

  • Asian dust events
  • Bacillus subtilis
  • Biosensor
  • Carbon nanotubes
  • Pathogen

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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