Development of high density mammalian cell culture system for the production of tissue-type plasminogen activator

Byong Gon Park, Joo Mi Chun, Chang Jin Lee, Gie Taek Chun, Ik Hwan Kim, Yeon Ho Jeong

Research output: Contribution to journalArticle

3 Citations (Scopus)


A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in situ removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis membrane and equipped on the agitator of spinner reactor for in situ removal of ammonium ion. The semi-fed-batch operation was employed to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Issue number2
Publication statusPublished - 2000 Jan 1



  • CHO cells
  • Microcarrier
  • Semi-fed-batch
  • Zeolite
  • in situ ammonium ion removal
  • tPA

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

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