Development of high density mammalian cell culture system for the production of tissue-type plasminogen activator

Byong Gon Park, Joo Mi Chun, Chang Jin Lee, Gie Taek Chun, Ik Hwan Kim, Yeon Ho Jeong

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination of in situ removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis membrane and equipped on the agitator of spinner reactor for in situ removal of ammonium ion. The semi-fed-batch operation was employed to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalBiotechnology and Bioprocess Engineering
Volume5
Issue number2
Publication statusPublished - 2000 Dec 1

Fingerprint

Tissue Plasminogen Activator
Ammonium Compounds
Cell culture
Cell Culture Techniques
Cell growth
Ions
Tissue
Dialysis membranes
Zeolites
CHO Cells
Growth
Glutamine
Glass
Glucose
Dialysis
Cell Count
Productivity
Membranes

Keywords

  • CHO cells
  • in situ ammonium ion removal
  • Microcarrier
  • Semi-fed-batch
  • tPA
  • Zeolite

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering

Cite this

Development of high density mammalian cell culture system for the production of tissue-type plasminogen activator. / Park, Byong Gon; Chun, Joo Mi; Lee, Chang Jin; Chun, Gie Taek; Kim, Ik Hwan; Jeong, Yeon Ho.

In: Biotechnology and Bioprocess Engineering, Vol. 5, No. 2, 01.12.2000, p. 123-129.

Research output: Contribution to journalArticle

Park, Byong Gon ; Chun, Joo Mi ; Lee, Chang Jin ; Chun, Gie Taek ; Kim, Ik Hwan ; Jeong, Yeon Ho. / Development of high density mammalian cell culture system for the production of tissue-type plasminogen activator. In: Biotechnology and Bioprocess Engineering. 2000 ; Vol. 5, No. 2. pp. 123-129.
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