Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin B1 in grain and feed samples

Won Bo Shim, Zheng You Yang, Jung Sook Kim, Ji Young Kim, Sung Jo Kang, Gun-Jo Woo, Young Chul Chung, Sergei A. Eremin, Duck Hwa Chung

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

Original languageEnglish
Pages (from-to)1629-1637
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume17
Issue number10
Publication statusPublished - 2007 Oct 1

Fingerprint

Immunochromatography
Aflatoxins
Aflatoxin B1
Antibodies
Gold
Screening
Agricultural products
Mycotoxins
Monoclonal antibodies
High Pressure Liquid Chromatography
Monoclonal Antibodies
Cross Reactions
Hybridomas
Limit of Detection
Costs
Costs and Cost Analysis
Food

Keywords

  • Aflatoxin B1
  • Immunochromatography strip test
  • Monoclonal antibody
  • Mycotoxins
  • Nanocolloidal gold

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin B1 in grain and feed samples. / Shim, Won Bo; Yang, Zheng You; Kim, Jung Sook; Kim, Ji Young; Kang, Sung Jo; Woo, Gun-Jo; Chung, Young Chul; Eremin, Sergei A.; Chung, Duck Hwa.

In: Journal of Microbiology and Biotechnology, Vol. 17, No. 10, 01.10.2007, p. 1629-1637.

Research output: Contribution to journalArticle

Shim, WB, Yang, ZY, Kim, JS, Kim, JY, Kang, SJ, Woo, G-J, Chung, YC, Eremin, SA & Chung, DH 2007, 'Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin B1 in grain and feed samples', Journal of Microbiology and Biotechnology, vol. 17, no. 10, pp. 1629-1637.
Shim, Won Bo ; Yang, Zheng You ; Kim, Jung Sook ; Kim, Ji Young ; Kang, Sung Jo ; Woo, Gun-Jo ; Chung, Young Chul ; Eremin, Sergei A. ; Chung, Duck Hwa. / Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin B1 in grain and feed samples. In: Journal of Microbiology and Biotechnology. 2007 ; Vol. 17, No. 10. pp. 1629-1637.
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