Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin B1 in grain and feed samples

Won Bo Shim, Zheng You Yang, Jung Sook Kim, Ji Young Kim, Sung Jo Kang, Gun Jo Woo, Young Chul Chung, Sergei A. Eremin, Duck Hwa Chung

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

Original languageEnglish
Pages (from-to)1629-1637
Number of pages9
JournalJournal of microbiology and biotechnology
Volume17
Issue number10
Publication statusPublished - 2007 Oct

Keywords

  • Aflatoxin B1
  • Immunochromatography strip test
  • Monoclonal antibody
  • Mycotoxins
  • Nanocolloidal gold

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin B1 in grain and feed samples'. Together they form a unique fingerprint.

  • Cite this

    Shim, W. B., Yang, Z. Y., Kim, J. S., Kim, J. Y., Kang, S. J., Woo, G. J., Chung, Y. C., Eremin, S. A., & Chung, D. H. (2007). Development of immunochromatography strip-test using nanocolloidal gold-antibody probe for the rapid detection of aflatoxin B1 in grain and feed samples. Journal of microbiology and biotechnology, 17(10), 1629-1637.