Development of individual plasmonic nanosensors for clinical diagnosis

Phuoc Long Truong, Sang Jun Sim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Accurate detection of panels of protein biomarkers in serum, saliva, or tissue plays an important role in early detection of diseases. Recently, our lab has developed the localized surface plasmon resonance (LSPR)-based immunosensors with highly promising analytical approach for qualification and quantification of biochemical substances in various applications. The LSPR of noble nanoparticles shifts upon refractive index changes of the dielectric environment surrounding the nanoparticles, subsequently causing a peak shift of the LSPR; it is also very sensitive to the binding events near the nanoparticle surface. Therefore, individual nanoparticles are sufficiently sensitive to detect the molecular interactions on the nanoparticle surface induced by biological molecules at ultralow concentrations. Furthermore, LSPR λmax shifts induced by adsorbates on the nanoparticle surface are completely linear with the number of bound molecules. Owing to this reason, each nanoparticle can be used as an independent sensor. By functionalizing the nanoparticle surface with the appropriate receptors, individual nanoparticle sensors can be used as diagnostic tools for a variety of diseases. Herein, we introduce the resonant Rayleigh scattering properties of single Au nanoparticles and the use of single Au nanoparticles as the plasmonic transducers to detect the binding of protein biomarkers to specific receptors conjugated gold nanoparticles. The principle of detection based on the resonant Rayleigh scattering response of single Au nanoparticles and the LSPR λmax shift of resonant Rayleigh scattering spectrum. This approach holds great promise as a simple, label-free, ultrasensitive method for detection of protein biomarkers in clinical diagnostics.

Original languageEnglish
Title of host publicationIFMBE Proceedings
PublisherSpringer Verlag
Pages1-6
Number of pages6
Volume46
ISBN (Print)9783319117751
DOIs
Publication statusPublished - 2015
Event5th International Conference on the Development of Biomedical Engineering, 2014 - Ho Chi Minh City, Viet Nam
Duration: 2014 Jun 162014 Jun 18

Other

Other5th International Conference on the Development of Biomedical Engineering, 2014
CountryViet Nam
CityHo Chi Minh City
Period14/6/1614/6/18

Keywords

  • Au nanoparticles (AuNPs)
  • Localized surface plasmon resonance (LSPR)
  • Plasmonic nanosensors
  • Rayleigh light scattering (RLS)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

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