Development of magnetic torque stimulation (Mts) utilizing rotating uniform magnetic field for mechanical activation of cardiac cells

Myeongjin Song, Jongseong Kim, Hyundo Shin, Yekwang Kim, Hwanseok Jang, Yongdoo Park, Seung Jong Kim

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Regulation of cell signaling through physical stimulation is an emerging topic in biomedicine. Background: While recent advances in biophysical technologies show capabilities for spatiotemporal stimulation, interfacing those tools with biological systems for intact signal transfer and noncontact stimulation remains challenging. Here, we describe the use of a magnetic torque stimulation (MTS) system combined with engineered magnetic particles to apply forces on the surface of individual cells. MTS utilizes an externally rotating magnetic field to induce a spin on magnetic particles and generate torsional force to stimulate mechanotransduction pathways in two types of human heart cells—cardiomyocytes and cardiac fibroblasts. Methods: The MTS system operates in a noncontact mode with two magnets separated (60 mm) from each other and generates a torque of up to 15 pN µm across the entire area of a 35-mm cell culture dish. The MTS system can mechanically stimulate both types of human heart cells, inducing maturation and hypertrophy. Results: Our findings show that application of the MTS system under hypoxic conditions induces not only nuclear localization of mechanoresponsive YAP proteins in human heart cells but also overexpression of hypertrophy markers, including β-myosin heavy chain (βMHC), cardiotrophin-1 (CT-1), microRNA-21 (miR-21), and transforming growth factor beta-1 (TGFβ-1). Conclusions: These results have important implications for the applicability of the MTS system to diverse in vitro studies that require remote and noninvasive mechanical regulation.

Original languageEnglish
Article number1684
Pages (from-to)1-13
Number of pages13
JournalNanomaterials
Volume10
Issue number9
DOIs
Publication statusPublished - 2020 Sep

Keywords

  • Cardiac cells
  • Hypoxia
  • Magnetogenetics
  • Mechanotransduction
  • Torsional magnetic stimulation

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Materials Science(all)

Fingerprint

Dive into the research topics of 'Development of magnetic torque stimulation (Mts) utilizing rotating uniform magnetic field for mechanical activation of cardiac cells'. Together they form a unique fingerprint.

Cite this