Development of magnetically separable immobilized lipase by using cellulose derivatives and their application in enantioselective esterification of ibuprofen

Gowoun Lee, Hongil Joo, Jungbae Kim, Jung Heon Lee

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Highly active, stable, and magnetically separable immobilized enzymes were developed using carboxymethyl cellulose (CMC) and diethylaminoethyl cellulose DEAE-C; hereafter designated "DEAE" as supporting materials. Iron oxide nanoparticles penetrated the micropores of the supporting materials, rendering them magnetically separable. Lipase (LP) was immobilized on the surface of the supporting materials by using cross-linked enzyme aggregation (CLEA) by glutaraldehyde. The activity of enzyme aggregates coated on DEAE was approximately 2 times higher than that of enzyme aggregates coated on CMC. This is explained by the fact that enzyme aggregates with amine residues are more efficient than those with carboxyl residues. After a 96-h enantioselective ibuprofen esterification reaction, 6% ibuprofen propyl ester was produced from the racemic mixture of ibuprofen by using DEAE-LP, and 2.8% using CMC-LP.

Original languageEnglish
Pages (from-to)465-471
Number of pages7
JournalJournal of microbiology and biotechnology
Issue number3
Publication statusPublished - 2008 Mar 28



  • Enzyme stabilization
  • Ibuprofen
  • Racemic resolution

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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