Development of magnetically separable polyaniline nanofibers for enzyme immobilization and recovery

Gowoun Lee, Jungbae Kim, Jung heon Lee

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


Magnetically separable polyaniline nanofibers were synthesized using a mixture of aniline solution and iron oxide; the nanofibers comprised of crosslinked linear polymers that could be separated from the reaction solution by using magnets. The covalently attached and aggregated lipases retained a high degree of stability, were easily recovered from the enzyme solution, and could be used repeatedly. The enzyme activity of the dispersed magnetic nanofibers was more than 80% of the original activity even under room temperature and shaking conditions for 32 days. The highly active and stable immobilized lipase (LP) was then used in an enantioselective esterification reaction, and 18% of the prophilic ester of ibuprofen, a chirally selective compound, was synthesized from racemic ibuprofen following a reaction for 96 h.

Original languageEnglish
Pages (from-to)466-472
Number of pages7
JournalEnzyme and Microbial Technology
Issue number6
Publication statusPublished - 2008 May 5


  • Enzyme stabilization
  • Ibuprofen
  • Racemic resolution

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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