Development of method for analysis of four sulfonylurea pesticides, rimsulfuron, ethametsulfuron-methyl, tribenuron-methyl, chlorimuron-ethyl residues by high-performance liquid chromatography with diode-array detection

Yun Chang Koo, Sung Yong Yang, Zeng Wang, Eun Mi An, Kyoung Heo, Hyengkook Kim, Han Seung Shin, Jin Won Lee, Kwang Won Lee

Research output: Contribution to journalArticle

Abstract

The method for residue analysis of four sulfonylurea pesticides, rimsulfuron, ethametsulfuron-methyl, tribenuron- methyl and chlorimuron-ethyl was examined and analyzed by HPLC with ODS column (250 mm×4.6 mm, 5 μm diameter particle size) which was maintained at 35°C. Mobile phase consisted of solvent A (20 mM KH 2PO 4, pH 2.5) and solvent B (acetonitrile). Isocratic elution of the column with 45% solvent A and 55% solvent B at a flow rate of 1 mL/min resulted in retention times of 5.92, 6.54, 9.28, and 14.35 min for rimsulfuron, ethametsulfuron-methyl, tribenuron-methyl, and chlorimuron-ethyl, respectively. All injection volumes were 20 μL. The limit of quantitation was 0.02, 0.01, 0.001, and 0.004 mg/kg for rimsulfuron, ethametsulfuron-methyl, tribenuron-methyl, and chlorimuron-ethyl, respectively. Recovery rate test was performed with three farm products, rice, apple and soybean. Four sulfonylurea pesticides were spiked at concentrations of 0.05, 0.1 and 0.5 mg/kg. The recovery rates were ranged from 86.12% to 116.26% and the standard deviations of all experiments were within 10%.

Original languageEnglish
Pages (from-to)1231-1235
Number of pages5
JournalJournal of the Korean Society of Food Science and Nutrition
Volume39
Issue number8
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

rimsulfuron
tribenuron
sulfonylureas
Pesticides
pesticides
high performance liquid chromatography
High Pressure Liquid Chromatography
Malus
agricultural products
methodology
Soybeans
Particle Size
particle size
apples
soybeans
injection
rice
Injections
chlorimuron ethyl
metsulfuron methyl

Keywords

  • HPLC
  • Pesticide
  • Residue analysis
  • Sulfonylurea

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science

Cite this

Development of method for analysis of four sulfonylurea pesticides, rimsulfuron, ethametsulfuron-methyl, tribenuron-methyl, chlorimuron-ethyl residues by high-performance liquid chromatography with diode-array detection. / Koo, Yun Chang; Yang, Sung Yong; Wang, Zeng; An, Eun Mi; Heo, Kyoung; Kim, Hyengkook; Shin, Han Seung; Lee, Jin Won; Lee, Kwang Won.

In: Journal of the Korean Society of Food Science and Nutrition, Vol. 39, No. 8, 01.12.2010, p. 1231-1235.

Research output: Contribution to journalArticle

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abstract = "The method for residue analysis of four sulfonylurea pesticides, rimsulfuron, ethametsulfuron-methyl, tribenuron- methyl and chlorimuron-ethyl was examined and analyzed by HPLC with ODS column (250 mm×4.6 mm, 5 μm diameter particle size) which was maintained at 35°C. Mobile phase consisted of solvent A (20 mM KH 2PO 4, pH 2.5) and solvent B (acetonitrile). Isocratic elution of the column with 45{\%} solvent A and 55{\%} solvent B at a flow rate of 1 mL/min resulted in retention times of 5.92, 6.54, 9.28, and 14.35 min for rimsulfuron, ethametsulfuron-methyl, tribenuron-methyl, and chlorimuron-ethyl, respectively. All injection volumes were 20 μL. The limit of quantitation was 0.02, 0.01, 0.001, and 0.004 mg/kg for rimsulfuron, ethametsulfuron-methyl, tribenuron-methyl, and chlorimuron-ethyl, respectively. Recovery rate test was performed with three farm products, rice, apple and soybean. Four sulfonylurea pesticides were spiked at concentrations of 0.05, 0.1 and 0.5 mg/kg. The recovery rates were ranged from 86.12{\%} to 116.26{\%} and the standard deviations of all experiments were within 10{\%}.",
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AU - Koo, Yun Chang

AU - Yang, Sung Yong

AU - Wang, Zeng

AU - An, Eun Mi

AU - Heo, Kyoung

AU - Kim, Hyengkook

AU - Shin, Han Seung

AU - Lee, Jin Won

AU - Lee, Kwang Won

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