Development of molecular markers for bentazone-resistant wheat (Triticum aestivum L.) induced by gamma-ray irradiation

J. Y. Kim, D. Y. Kim, C. S. Jang, Yong Weon Seo, D. S. Kim, J. B. Kim, K. R. Kim

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Abstract

Wheat mutants generated from a cultivar "Geumgangmil" by using a 60Co source (gamma-ray) were developed for bentazone (3-isopropyl-2,1,3-benzo-thiadiazin-4-one-2,2-dioxide) resistance. Resistant M2 plants were selected from seedlings and adult plants consecutively. Bentazone-resistant wheat plants were able to survive at up to 10 times (1,600 ppm) the commercially recommended concentration. The four plants with the highest bentazone resistance were selected. An AFLP (amplified fragment length polymorphism) analysis was applied to identify molecular markers for bentazone-resistant wheat mutants. Twelve polymorphic products, which were unique to bentazone-resistant plants, were identified, cloned, and sequenced. Among the twelve polymorphic bands, eight Sequence-tagged Sites (STS) primer sets were designed. Only one STS primer set (HRMW-08) was converted a "Bentazone-resistant wheat-specific" STS marker. The wheat mutants and markers that we obtained could be employed in a cross-breeding program incorporated with marker assisted selection (MAS) in the early stages of population segregation.

Original languageEnglish
Pages (from-to)1499-1505
Number of pages7
JournalJournal of the Korean Physical Society
Volume50
Issue number5 PART I
Publication statusPublished - 2007 May 1

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Keywords

  • AFLP marker
  • Bentazone-resistant wheat
  • Gamma-ray irradiation
  • MAS (marker assisted selection)
  • STS marker

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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