TY - JOUR
T1 - Development of multi-well-based electrochemical dissolved oxygen sensor array
AU - Han, Ji Hoon
AU - Kim, Saeyoung
AU - Choi, Jaesung
AU - Kang, Sora
AU - Pak, Youngmi Kim
AU - Pak, James Jungho
N1 - Funding Information:
This work was supported by the R&D program of MOTIE/KEIT. [10054488, Development of cellular metabolic rate analyzer that can simultaneously measure multiple parameters (pH, dissolved oxygen, and metabolic heat) from less than 1,000 cells]
Funding Information:
This work was supported by the R&D program of MOTIE/KEIT . [10054488, Development of cellular metabolic rate analyzer that can simultaneously measure multiple parameters (pH, dissolved oxygen, and metabolic heat) from less than 1,000 cells] Appendix A
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/3/1
Y1 - 2020/3/1
N2 - This paper describes a multi-well-based (24-well) electrochemical dissolved oxygen sensor (m-well ECDO sensor) array on a glass substrate, using Nafion as a solid polymer electrolyte. The designed m- well ECDO sensor array shares working electrodes, reference electrodes, and counter electrodes, to reduce the number of electrode pads to approximately one-fourth of its expected pad numbers (72 to 18), compared to the simply repeated 24 sensors. This m-well ECDO sensor array can simultaneously measure up to six sensors at individual crossing points of the row and column electrodes. The fabricated m-well ECDO sensor array showed good repeatability during five consecutive measurements at intervals of 100 s with a 2.4 μm thick Nafion membrane. The response time was about 10 s when the solution state changed from the air-saturated state to the zero-oxygen state, and the measured output current was about -1 μA and-20 nA in the corresponding states, respectively. The fabricated m-well ECDO sensor array was applied to measure the oxygen consumption rate of C2C12 cells for a cell metabolic study. The ratios of the uncoupled oxygen consumption rate (OCR) to the coupled OCR obtained from three sensors of the fabricated m-well ECDO sensor array were 2.16, 2.22, 2.18, which are higher than 1.71, 1.68, 1.73 obtained from of the XF-24 of Agilent Inc. in 5,000, 10,000, 30,000 cells, respectively. This indicates that using the fabricated m-well ECDO sensor array, variations in DO (dissolved oxygen) concentration due to cellular respiration can be effectively detected, even at low cell numbers.
AB - This paper describes a multi-well-based (24-well) electrochemical dissolved oxygen sensor (m-well ECDO sensor) array on a glass substrate, using Nafion as a solid polymer electrolyte. The designed m- well ECDO sensor array shares working electrodes, reference electrodes, and counter electrodes, to reduce the number of electrode pads to approximately one-fourth of its expected pad numbers (72 to 18), compared to the simply repeated 24 sensors. This m-well ECDO sensor array can simultaneously measure up to six sensors at individual crossing points of the row and column electrodes. The fabricated m-well ECDO sensor array showed good repeatability during five consecutive measurements at intervals of 100 s with a 2.4 μm thick Nafion membrane. The response time was about 10 s when the solution state changed from the air-saturated state to the zero-oxygen state, and the measured output current was about -1 μA and-20 nA in the corresponding states, respectively. The fabricated m-well ECDO sensor array was applied to measure the oxygen consumption rate of C2C12 cells for a cell metabolic study. The ratios of the uncoupled oxygen consumption rate (OCR) to the coupled OCR obtained from three sensors of the fabricated m-well ECDO sensor array were 2.16, 2.22, 2.18, which are higher than 1.71, 1.68, 1.73 obtained from of the XF-24 of Agilent Inc. in 5,000, 10,000, 30,000 cells, respectively. This indicates that using the fabricated m-well ECDO sensor array, variations in DO (dissolved oxygen) concentration due to cellular respiration can be effectively detected, even at low cell numbers.
KW - Cell metabolism
KW - Dissolved oxygen sensor
KW - Electrochemical sensor
KW - Multi-well based sensor array
KW - Oxygen consumption rate
UR - http://www.scopus.com/inward/record.url?scp=85076540419&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2019.127465
DO - 10.1016/j.snb.2019.127465
M3 - Article
AN - SCOPUS:85076540419
VL - 306
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
SN - 0925-4005
M1 - 127465
ER -