Development of multiplex reverse transcription polymerase chain reaction for detection and typing of parainfluenza viruses

Gu Choul Shin, Chan Park, Joo Yeon Lee, Byoung Kuk Na, Jong Won Park, Chun Kang, Jee Hee Kim, Woo Joo Kim, Chul Yong Song

Research output: Contribution to journalArticle

Abstract

Multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) method was developed for the simultaneous detection and typing of parainfluenza viruses (PIVs) which are an important cause of respiratory morbidity. The primer pairs for the multiplex RT-PCR were designed based on the nucleotide sequences of hemagglutinin-neuraminidase (HN) coding region of the genomic RNA. Multiplex RT-PCR yielded specific products for each type of PIVs and did not show the cross reaction with other respiratory viruses, such as adenoviruses, respiratory syncytial viruses (RSV), and influenza viruses. In comparison with the immunofluorescence assay (IFA), the multiplex RT-PCR method was 100 times more sensitive than IFA. These results suggest that the multiplex RT-PCR may be useful for the specific and rapid detection and typing of PIVs 1, 2 and 3 in a single-tube reaction.

Original languageEnglish
Pages (from-to)199-206
Number of pages8
JournalJournal of Bacteriology and Virology
Volume31
Issue number2
Publication statusPublished - 2001 Jan 1
Externally publishedYes

Fingerprint

Paramyxoviridae Infections
Reverse Transcription
Viruses
Polymerase Chain Reaction
Fluorescent Antibody Technique
Respiratory Syncytial Viruses
Multiplex Polymerase Chain Reaction
Cross Reactions
Hemagglutinins
Neuraminidase
Orthomyxoviridae
Adenoviridae
RNA
Morbidity

Keywords

  • Detection
  • IFA
  • Multiplex RT-PCR
  • Parainfluenza virus
  • Typing

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Shin, G. C., Park, C., Lee, J. Y., Na, B. K., Park, J. W., Kang, C., ... Song, C. Y. (2001). Development of multiplex reverse transcription polymerase chain reaction for detection and typing of parainfluenza viruses. Journal of Bacteriology and Virology, 31(2), 199-206.

Development of multiplex reverse transcription polymerase chain reaction for detection and typing of parainfluenza viruses. / Shin, Gu Choul; Park, Chan; Lee, Joo Yeon; Na, Byoung Kuk; Park, Jong Won; Kang, Chun; Kim, Jee Hee; Kim, Woo Joo; Song, Chul Yong.

In: Journal of Bacteriology and Virology, Vol. 31, No. 2, 01.01.2001, p. 199-206.

Research output: Contribution to journalArticle

Shin, GC, Park, C, Lee, JY, Na, BK, Park, JW, Kang, C, Kim, JH, Kim, WJ & Song, CY 2001, 'Development of multiplex reverse transcription polymerase chain reaction for detection and typing of parainfluenza viruses', Journal of Bacteriology and Virology, vol. 31, no. 2, pp. 199-206.
Shin, Gu Choul ; Park, Chan ; Lee, Joo Yeon ; Na, Byoung Kuk ; Park, Jong Won ; Kang, Chun ; Kim, Jee Hee ; Kim, Woo Joo ; Song, Chul Yong. / Development of multiplex reverse transcription polymerase chain reaction for detection and typing of parainfluenza viruses. In: Journal of Bacteriology and Virology. 2001 ; Vol. 31, No. 2. pp. 199-206.
@article{0d4e0b91bb2d4f9cb113347df0282b26,
title = "Development of multiplex reverse transcription polymerase chain reaction for detection and typing of parainfluenza viruses",
abstract = "Multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) method was developed for the simultaneous detection and typing of parainfluenza viruses (PIVs) which are an important cause of respiratory morbidity. The primer pairs for the multiplex RT-PCR were designed based on the nucleotide sequences of hemagglutinin-neuraminidase (HN) coding region of the genomic RNA. Multiplex RT-PCR yielded specific products for each type of PIVs and did not show the cross reaction with other respiratory viruses, such as adenoviruses, respiratory syncytial viruses (RSV), and influenza viruses. In comparison with the immunofluorescence assay (IFA), the multiplex RT-PCR method was 100 times more sensitive than IFA. These results suggest that the multiplex RT-PCR may be useful for the specific and rapid detection and typing of PIVs 1, 2 and 3 in a single-tube reaction.",
keywords = "Detection, IFA, Multiplex RT-PCR, Parainfluenza virus, Typing",
author = "Shin, {Gu Choul} and Chan Park and Lee, {Joo Yeon} and Na, {Byoung Kuk} and Park, {Jong Won} and Chun Kang and Kim, {Jee Hee} and Kim, {Woo Joo} and Song, {Chul Yong}",
year = "2001",
month = "1",
day = "1",
language = "English",
volume = "31",
pages = "199--206",
journal = "Journal of Bacteriology and Virology",
issn = "1598-2467",
publisher = "Chonnam National University Medical School",
number = "2",

}

TY - JOUR

T1 - Development of multiplex reverse transcription polymerase chain reaction for detection and typing of parainfluenza viruses

AU - Shin, Gu Choul

AU - Park, Chan

AU - Lee, Joo Yeon

AU - Na, Byoung Kuk

AU - Park, Jong Won

AU - Kang, Chun

AU - Kim, Jee Hee

AU - Kim, Woo Joo

AU - Song, Chul Yong

PY - 2001/1/1

Y1 - 2001/1/1

N2 - Multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) method was developed for the simultaneous detection and typing of parainfluenza viruses (PIVs) which are an important cause of respiratory morbidity. The primer pairs for the multiplex RT-PCR were designed based on the nucleotide sequences of hemagglutinin-neuraminidase (HN) coding region of the genomic RNA. Multiplex RT-PCR yielded specific products for each type of PIVs and did not show the cross reaction with other respiratory viruses, such as adenoviruses, respiratory syncytial viruses (RSV), and influenza viruses. In comparison with the immunofluorescence assay (IFA), the multiplex RT-PCR method was 100 times more sensitive than IFA. These results suggest that the multiplex RT-PCR may be useful for the specific and rapid detection and typing of PIVs 1, 2 and 3 in a single-tube reaction.

AB - Multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) method was developed for the simultaneous detection and typing of parainfluenza viruses (PIVs) which are an important cause of respiratory morbidity. The primer pairs for the multiplex RT-PCR were designed based on the nucleotide sequences of hemagglutinin-neuraminidase (HN) coding region of the genomic RNA. Multiplex RT-PCR yielded specific products for each type of PIVs and did not show the cross reaction with other respiratory viruses, such as adenoviruses, respiratory syncytial viruses (RSV), and influenza viruses. In comparison with the immunofluorescence assay (IFA), the multiplex RT-PCR method was 100 times more sensitive than IFA. These results suggest that the multiplex RT-PCR may be useful for the specific and rapid detection and typing of PIVs 1, 2 and 3 in a single-tube reaction.

KW - Detection

KW - IFA

KW - Multiplex RT-PCR

KW - Parainfluenza virus

KW - Typing

UR - http://www.scopus.com/inward/record.url?scp=24044451324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24044451324&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:24044451324

VL - 31

SP - 199

EP - 206

JO - Journal of Bacteriology and Virology

JF - Journal of Bacteriology and Virology

SN - 1598-2467

IS - 2

ER -