Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration

Yun Gyong Ahn, Jungju Seo, Jeoung Hwa Shin, Jeehyeong Khim, Jongki Hong

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Freezing-lipid filtration as a new method has been developed for the rapid determination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) in biological samples. This method can effectively reduce the time of sample pretreatment, labor and amount of solvents compared with conventional methods. By freezing-lipid filtration procedure, about 90% of lipids in extract could be removed without any significant loss of PCDD/Fs. For further cleanup of extracts after freezing-lipid filtration, automatic parallel LC columns including silica gel, alumina and carbon columns were applied. During automatic parallel LC columns cleanup, most of co-extracted interferences such as residue lipids and fatty acids could be eliminated and dioxins could be separated from many other dioxin-like congeners such as polychlorinated biphenyls by this procedure. The extracts after cleanup were analyzed by high-resolution gas chromatography (HRGC)/high-resolution mass spectrometry (HRMS) using an isotope dilution method. The average recoveries and relative standard deviation (R.S.D.) of 17 native congeners in the spiked fish samples at 8-80 pg/g (n = 3) were ranged between 85.3 and 117.2% and 5.7-20.3%, respectively.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalAnalytica Chimica Acta
Volume576
Issue number1
DOIs
Publication statusPublished - 2006 Aug 18

Fingerprint

dibenzofuran
cleanup
Freezing
dioxin
Fish
freezing
Fishes
lipid
Lipids
fish
Dioxins
Aluminum Oxide
Polychlorinated Biphenyls
Silica Gel
Distillation columns
PCDD
Isotopes
Gas chromatography
aluminum oxide
Dilution

Keywords

  • Automatic parallel LC
  • Fish
  • Freezing-lipid filtration
  • HRGC/HRMS
  • Polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs)

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration. / Ahn, Yun Gyong; Seo, Jungju; Shin, Jeoung Hwa; Khim, Jeehyeong; Hong, Jongki.

In: Analytica Chimica Acta, Vol. 576, No. 1, 18.08.2006, p. 31-36.

Research output: Contribution to journalArticle

Ahn, Yun Gyong ; Seo, Jungju ; Shin, Jeoung Hwa ; Khim, Jeehyeong ; Hong, Jongki. / Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration. In: Analytica Chimica Acta. 2006 ; Vol. 576, No. 1. pp. 31-36.
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