Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration

Yun Gyong Ahn, Jungju Seo, Jeoung Hwa Shin, Jeehyeong Khim, Jongki Hong

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Freezing-lipid filtration as a new method has been developed for the rapid determination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) in biological samples. This method can effectively reduce the time of sample pretreatment, labor and amount of solvents compared with conventional methods. By freezing-lipid filtration procedure, about 90% of lipids in extract could be removed without any significant loss of PCDD/Fs. For further cleanup of extracts after freezing-lipid filtration, automatic parallel LC columns including silica gel, alumina and carbon columns were applied. During automatic parallel LC columns cleanup, most of co-extracted interferences such as residue lipids and fatty acids could be eliminated and dioxins could be separated from many other dioxin-like congeners such as polychlorinated biphenyls by this procedure. The extracts after cleanup were analyzed by high-resolution gas chromatography (HRGC)/high-resolution mass spectrometry (HRMS) using an isotope dilution method. The average recoveries and relative standard deviation (R.S.D.) of 17 native congeners in the spiked fish samples at 8-80 pg/g (n = 3) were ranged between 85.3 and 117.2% and 5.7-20.3%, respectively.

Original languageEnglish
Pages (from-to)31-36
Number of pages6
JournalAnalytica Chimica Acta
Volume576
Issue number1
DOIs
Publication statusPublished - 2006 Aug 18

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Keywords

  • Automatic parallel LC
  • Fish
  • Freezing-lipid filtration
  • HRGC/HRMS
  • Polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs)

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

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