Abstract
Freezing-lipid filtration as a new method has been developed for the rapid determination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) in biological samples. This method can effectively reduce the time of sample pretreatment, labor and amount of solvents compared with conventional methods. By freezing-lipid filtration procedure, about 90% of lipids in extract could be removed without any significant loss of PCDD/Fs. For further cleanup of extracts after freezing-lipid filtration, automatic parallel LC columns including silica gel, alumina and carbon columns were applied. During automatic parallel LC columns cleanup, most of co-extracted interferences such as residue lipids and fatty acids could be eliminated and dioxins could be separated from many other dioxin-like congeners such as polychlorinated biphenyls by this procedure. The extracts after cleanup were analyzed by high-resolution gas chromatography (HRGC)/high-resolution mass spectrometry (HRMS) using an isotope dilution method. The average recoveries and relative standard deviation (R.S.D.) of 17 native congeners in the spiked fish samples at 8-80 pg/g (n = 3) were ranged between 85.3 and 117.2% and 5.7-20.3%, respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 31-36 |
| Number of pages | 6 |
| Journal | Analytica Chimica Acta |
| Volume | 576 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 Aug 18 |
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Keywords
- Automatic parallel LC
- Fish
- Freezing-lipid filtration
- HRGC/HRMS
- Polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs)
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration. / Ahn, Yun Gyong; Seo, Jungju; Shin, Jeoung Hwa; Khim, Jeehyeong; Hong, Jongki.
In: Analytica Chimica Acta, Vol. 576, No. 1, 18.08.2006, p. 31-36.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of new cleanup method of polychlorinated dibenzo-p-dioxins/dibenzofurans in fish by freezing-lipid filtration
AU - Ahn, Yun Gyong
AU - Seo, Jungju
AU - Shin, Jeoung Hwa
AU - Khim, Jeehyeong
AU - Hong, Jongki
PY - 2006/8/18
Y1 - 2006/8/18
N2 - Freezing-lipid filtration as a new method has been developed for the rapid determination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) in biological samples. This method can effectively reduce the time of sample pretreatment, labor and amount of solvents compared with conventional methods. By freezing-lipid filtration procedure, about 90% of lipids in extract could be removed without any significant loss of PCDD/Fs. For further cleanup of extracts after freezing-lipid filtration, automatic parallel LC columns including silica gel, alumina and carbon columns were applied. During automatic parallel LC columns cleanup, most of co-extracted interferences such as residue lipids and fatty acids could be eliminated and dioxins could be separated from many other dioxin-like congeners such as polychlorinated biphenyls by this procedure. The extracts after cleanup were analyzed by high-resolution gas chromatography (HRGC)/high-resolution mass spectrometry (HRMS) using an isotope dilution method. The average recoveries and relative standard deviation (R.S.D.) of 17 native congeners in the spiked fish samples at 8-80 pg/g (n = 3) were ranged between 85.3 and 117.2% and 5.7-20.3%, respectively.
AB - Freezing-lipid filtration as a new method has been developed for the rapid determination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs) in biological samples. This method can effectively reduce the time of sample pretreatment, labor and amount of solvents compared with conventional methods. By freezing-lipid filtration procedure, about 90% of lipids in extract could be removed without any significant loss of PCDD/Fs. For further cleanup of extracts after freezing-lipid filtration, automatic parallel LC columns including silica gel, alumina and carbon columns were applied. During automatic parallel LC columns cleanup, most of co-extracted interferences such as residue lipids and fatty acids could be eliminated and dioxins could be separated from many other dioxin-like congeners such as polychlorinated biphenyls by this procedure. The extracts after cleanup were analyzed by high-resolution gas chromatography (HRGC)/high-resolution mass spectrometry (HRMS) using an isotope dilution method. The average recoveries and relative standard deviation (R.S.D.) of 17 native congeners in the spiked fish samples at 8-80 pg/g (n = 3) were ranged between 85.3 and 117.2% and 5.7-20.3%, respectively.
KW - Automatic parallel LC
KW - Fish
KW - Freezing-lipid filtration
KW - HRGC/HRMS
KW - Polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDDs/Fs)
UR - http://www.scopus.com/inward/record.url?scp=33746886287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33746886287&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2006.03.010
DO - 10.1016/j.aca.2006.03.010
M3 - Article
C2 - 17723610
AN - SCOPUS:33746886287
VL - 576
SP - 31
EP - 36
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1
ER -