Development of non-pathogenic bacterial biofilms on the surface of stainless steel which are inhibitory to Salmonella enterica

Yoonbin Kim, Hoikyung Kim, Larry R. Beuchat, Jee-Hoon Ryu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Non-pathogenic bacterial biofilms were developed on the surface of stainless steel possessing desiccation tolerance and antimicrobial activity against Salmonella enterica. Three bacteria exhibiting strong antimicrobial activities against S. enterica were isolated from various soils, foods, and food-contact surfaces. Isolates were identified as Pseudomonas extremorientalis (strain Lettuce-28), Paenibacillus peoriae (strain Lettuce-7), and Streptomyces cirratus (strain Geumsan-207). These bacteria grew rapidly and formed biofilms within 24 h on the surface of stainless steel coupons (SSCs) immersed in laboratory media (tryptic soy broth or Bennet's broth) at 25 °C. Cells in biofilms had enhanced tolerance to desiccation (exposure to 43% atmospheric relative humidity [RH]) and retained antimicrobial activity against S. enterica. Populations of S. enterica deposited on SSCs containing biofilm formed by Ps. extremorientalis strain Lettuce-28, for example, decreased by > 2.5 log CFU/coupon within 24 h at 25 °C and 43% RH, while the number of cells inoculated on SSCs lacking biofilm decreased by 1.5 log CFU/coupon. Antimicrobial activities of the three antagonistic bacteria against S. enterica persisted in desiccated biofilms. This study provides insights to developing strategies to inactivate Salmonella and perhaps other foodborne pathogens on abiotic surfaces using non-pathogenic antagonistic bacteria.

Original languageEnglish
Pages (from-to)136-142
Number of pages7
JournalFood Microbiology
Volume69
DOIs
Publication statusPublished - 2018 Feb 1

Fingerprint

Salmonella enterica
Stainless Steel
stainless steel
Biofilms
biofilm
Lettuce
anti-infective agents
lettuce
Bacteria
Desiccation
bacteria
Humidity
Paenibacillus peoriae
relative humidity
Paenibacillus
food contact surfaces
Food
Streptomyces
food pathogens
Pseudomonas

Keywords

  • Antagonistic bacteria
  • Biocontrol
  • Biofilm
  • Desiccation
  • Salmonella enterica
  • Stainless steel surface

ASJC Scopus subject areas

  • Microbiology
  • Food Science

Cite this

Development of non-pathogenic bacterial biofilms on the surface of stainless steel which are inhibitory to Salmonella enterica. / Kim, Yoonbin; Kim, Hoikyung; Beuchat, Larry R.; Ryu, Jee-Hoon.

In: Food Microbiology, Vol. 69, 01.02.2018, p. 136-142.

Research output: Contribution to journalArticle

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